PMID- 8245867
OWN - NLM
STAT- MEDLINE
DCOM- 19931227
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 74 ( Pt 11)
DP  - 1993 Nov
TI  - Characterization of an in vivo reactivation model of herpes simplex virus from 
      mice trigeminal ganglia.
PG  - 2487-91
AB  - Herpes simplex virus type 1 (HSV-1) is transcriptionally active during latent 
      infection in human peripheral sensory ganglia. Viral gene expression includes the 
      latency-associated transcripts (LATs) which have been linked to the ability of 
      the virus to resume replication and reactivate. However, the molecular basis of 
      reactivation and the mechanisms of action of these transcripts are unknown. In 
      order to study these parameters, an in vivo reactivation model is needed. We 
      investigated use of the mouse as the experimental animal, modifying the route of 
      infection, the viral strain and the reactivation protocol. Following 
      administration of human immunoglobulin 1 day prior to corneal infection, no 
      infectious virus was detected in trigeminal ganglia (TG). However, latency was 
      established in all infected animals as indicated by explant reactivation of TG, 
      and in vivo reactivation was achieved in 30 to 40% of them. DNA quantification 
      revealed that TG of immunized mice contained more HSV-1 DNA than did those of 
      non-immunized mice. By in situ hybridization twice as many neuronal cells in TG 
      of immunized mice were positive for LATs, compared with infected but 
      non-immunized, mice. These findings suggest that suppression of primary infection 
      facilitates reactivation by increasing HSV-1 copy number in latently infected 
      nervous tissue.
FAU - Birmanns, B
AU  - Birmanns B
AD  - Department of Neurology, Hadassah University Hospital, Jerusalem, Israel.
FAU - Reibstein, I
AU  - Reibstein I
FAU - Steiner, I
AU  - Steiner I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Cornea
MH  - Female
MH  - Herpes Simplex/immunology/microbiology
MH  - Immune Sera/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Models, Biological
MH  - Simplexvirus/*physiology
MH  - Trigeminal Ganglion/*microbiology
MH  - Virus Activation/immunology/*physiology
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 10.1099/0022-1317-74-11-2487 [doi]
PST - ppublish
SO  - J Gen Virol. 1993 Nov;74 ( Pt 11):2487-91. doi: 10.1099/0022-1317-74-11-2487.