PMID- 8249915
OWN - NLM
STAT- MEDLINE
DCOM- 19931228
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 100
IP  - 6
DP  - 1993 Dec
TI  - Identification of mismatched fixed specimens with a commercially available kit 
      based on the polymerase chain reaction.
PG  - 666-70
AB  - Specimen mix-ups inevitably occur and have the potential for great harm. The 
      ability to investigate mix-ups objectively and assign fixed tissues to patients 
      correctly is unfortunately limited, as most such assays require fresh specimens. 
      A commercial kit based on the polymerase chain reaction (PCR) can be applied to 
      the molecular genetic analysis of fixed tissues. This kit, which can amplify and 
      distinguish 21 different genotypes at a polymorphic human leukocyte antigen (HLA) 
      locus, was applied to investigate 16 cases of potential specimen mismatches. The 
      majority of tissues were small and essentially irreplaceable biopsy specimens, 
      and four cases involved minute fragments of potential "floaters." Data were 
      successfully obtained from all 16 cases despite the collection from several 
      different hospitals and the small quantities of tissue. The assay required 
      approximately 2 days for completion; therefore, data were returned within a 
      clinically useful time period. This study provided evidence that molecular 
      genetic assays based on the PCR can be applied to routinely obtained fixed-tissue 
      specimens to investigate potential mismatches.
FAU - Shibata, D
AU  - Shibata D
AD  - Department of Pathology, University of Southern California School of Medicine, 
      Los Angeles.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
CIN - Am J Clin Pathol. 1993 Dec;100(6):592-3. PMID: 8249903
MH  - DNA Fingerprinting
MH  - Humans
MH  - Pathology, Clinical/methods/*standards
MH  - *Polymerase Chain Reaction
MH  - *Quality Control
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1093/ajcp/100.6.666 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1993 Dec;100(6):666-70. doi: 10.1093/ajcp/100.6.666.