PMID- 8250538
OWN - NLM
STAT- MEDLINE
DCOM- 19940104
LR  - 20041117
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 34
IP  - 6
DP  - 1993 Dec
TI  - Demonstration of human T-cell lymphotropic virus type I (HTLV-I) from an HTLV-I 
      seronegative south Indian patient with chronic, progressive spastic paraparesis.
PG  - 867-70
AB  - Here we describe a human T-cell lymphotropic virus type I (HTLV-I) seronegative 
      patient from South India with a chronic, progressive spastic paraparesis from 
      which HTLV-I has been isolated from peripheral blood lymphocytes. HTLV-I pol and 
      tax viral sequences were detected in DNA from fresh peripheral blood lymphocytes 
      (PBL) by polymerase chain reaction (PCR) and liquid hybridization techniques. 
      Southern blot analysis of the PCR products demonstrated a low copy number of 
      HTLV-I at the level of one viral copy per 10,000 fresh PBL. A long-term CD4+ 
      T-cell line was established from PBL of this patient using recombinant 
      interleukin-2, OKT3, and feeder cells. DNA from these cultured lines was 
      amplified and portions of the HTLV-I long terminal repeat (U3), pol, env, and tax 
      regions were sequenced (a total of 1,115 bp). The sequence data showed that the 
      HTLV-I associated with this patient was 98.8% homologous to prototype HTLV-I. 
      Southern blot analysis also confirmed the presence of full-length HTLV-I. These 
      results indicate that HTLV-I can be demonstrated in an HTLV-I seronegative 
      patient from South India with a chronic progressive neurological disorder.
FAU - Nishimura, M
AU  - Nishimura M
AD  - Neuroimmunology Branch, National Institutes of Health, Bethesda, MD 20892.
FAU - Mingioli, E
AU  - Mingioli E
FAU - McFarlin, D E
AU  - McFarlin DE
FAU - Jacobson, S
AU  - Jacobson S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
SB  - IM
MH  - Base Sequence
MH  - Chronic Disease
MH  - Human T-lymphotropic virus 1/genetics/*isolation & purification
MH  - Humans
MH  - India
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/*microbiology
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1002/ana.410340618 [doi]
PST - ppublish
SO  - Ann Neurol. 1993 Dec;34(6):867-70. doi: 10.1002/ana.410340618.