PMID- 8252391
OWN - NLM
STAT- MEDLINE
DCOM- 19940113
LR  - 20220309
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 624
IP  - 1-2
DP  - 1993 Oct 8
TI  - Topography of serotonin neurons in the dorsal raphe nucleus that send axon 
      collaterals to the rat prefrontal cortex and nucleus accumbens.
PG  - 188-98
AB  - Diverse physiological actions have been reported for 5-hydroxytryptamine (5-HT, 
      serotonin) in the medial prefrontal cortex (MPFC) and the nucleus accumbens (Acb) 
      suggesting that the 5-HT innervation of these forebrain areas may be derived from 
      different populations of neurons. We examined this possibility by mapping the 
      distribution of 5-HT-immunoreactive (ir) and non-5-HT-ir neurons containing 
      retrograde labeling following injections of different tracers into both these 
      target regions. The analysis was focused in the dorsal raphe nucleus (DRN) of the 
      midbrain, since 5-HT pathways to the MPFC and Acb primarily originate from this 
      area. Volume microinjections of the fluorescent retrograde tracer, Fluoro-Gold 
      (FG), were placed into the MPFC and microinjections of cholera toxin B subunit 
      coupled to 15 nm gold particles (CT-Au) were placed into the Acb of the same 
      animal. Sections through the DRN containing retrogradely labeled neurons were 
      further processed for immunofluorescent localization of 5-HT using a rhodamine 
      marker. Neurons retrogradely labeled from the Acb were greater in number overall 
      than those projecting to the MPFC. In addition, Acb-projecting neurons extended 
      into the lateral wings of the DRN, whereas MPFC-projecting neurons were more 
      restricted to the midline. Both groups of retrogradely labeled neurons, however, 
      were more numerous in the caudal aspect of the dorsal raphe nucleus and were 
      scattered amongst 5-HT immunoreactive perikarya. Of 783 +/- 69 CT-Au labeled 
      cells, 15% also contained the FG label and 11% contained FG and 5-HT 
      immunoreactivity. Of 613 +/- 48 FG labeled cells, 24% also contained the CT-Au 
      label and 21% were also immunoreactive to 5-HT. The results suggest a more 
      prominent input to the Acb from both 5-HT-ir and non-5-HT-ir neurons in the 
      caudal aspect of the DRN and further indicate that while most 5-HT-ir and 
      non-5-HT-ir neurons project differentially to both forebrain regions, a few cells 
      also show collateralization to the MPFC and Acb. Such collateralization of single 
      serotonergic neurons to divergent targets may integrate cognitive and motor 
      activities in response to pharmacological manipulations of ascending serotonergic 
      pathways.
FAU - Van Bockstaele, E J
AU  - Van Bockstaele EJ
AD  - Department of Neurobiology, Cornell University Medical College, New York, NY 
      10021.
FAU - Biswas, A
AU  - Biswas A
FAU - Pickel, V M
AU  - Pickel VM
LA  - eng
GR  - 5F32 NS09100-02/NS/NINDS NIH HHS/United States
GR  - NIMH MH00078/MH/NIMH NIH HHS/United States
GR  - NIMH MH40342/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - Immunohistochemistry
MH  - Male
MH  - Neurons/*metabolism/physiology
MH  - Nucleus Accumbens/*physiology
MH  - Prefrontal Cortex/*physiology
MH  - Raphe Nuclei/cytology/*metabolism/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/*metabolism
MH  - Synaptic Transmission
EDAT- 1993/10/08 00:00
MHDA- 1993/10/08 00:01
CRDT- 1993/10/08 00:00
PHST- 1993/10/08 00:00 [pubmed]
PHST- 1993/10/08 00:01 [medline]
PHST- 1993/10/08 00:00 [entrez]
AID - 0006-8993(93)90077-Z [pii]
AID - 10.1016/0006-8993(93)90077-z [doi]
PST - ppublish
SO  - Brain Res. 1993 Oct 8;624(1-2):188-98. doi: 10.1016/0006-8993(93)90077-z.