PMID- 8253850
OWN - NLM
STAT- MEDLINE
DCOM- 19940113
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 123
IP  - 6 Pt 1
DP  - 1993 Dec
TI  - Ectopic trkA expression mediates a NGF survival response in NGF-independent 
      sensory neurons but not in parasympathetic neurons.
PG  - 1555-66
AB  - We have investigated the role of trkA, the tyrosine kinase NGF receptor, in 
      mediating the survival response of embryonic neurons to NGF. Embryonic trigeminal 
      mesencephalic (TMN) neurons, which normally survive in the presence of 
      brain-derived neurotrophic factor (BDNF) but not NGF, become NGF-responsive when 
      microinjected with an expression vector containing trkA cDNA. In contrast, 
      microinjection of ciliary neurotrophic factor (CNTF)-dependent embryonic ciliary 
      neurons with the same construct does not result in the acquisition of NGF 
      responsiveness by these neurons despite de novo expression of trkA mRNA and 
      protein. The failure of trkA to result in an NGF-promoted survival response in 
      ciliary neurons is not due to absence of the low-affinity NGF receptor, p75, in 
      these neurons. Quantitative RT/PCR and immunocytochemistry showed that TMN and 
      ciliary neurons both express p75 mRNA and protein. These findings not only 
      provide the first direct experimental demonstration of trkA mediating a 
      physiological response in an appropriate cell type, namely NGF-promoted survival 
      of embryonic neurons, but indicate that not all neurons are able to respond to a 
      trkA-mediated signal transduction event.
FAU - Allsopp, T E
AU  - Allsopp TE
AD  - School of Biological and Medical Sciences, St. Andrews University, Fife, United 
      Kingdom.
FAU - Robinson, M
AU  - Robinson M
FAU - Wyatt, S
AU  - Wyatt S
FAU - Davies, A M
AU  - Davies AM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (DNA Primers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
GS  - trkA
MH  - Animals
MH  - Base Sequence
MH  - Cell Survival
MH  - Chick Embryo
MH  - DNA Primers/chemistry
MH  - Ganglia, Parasympathetic/*cytology
MH  - Gene Expression
MH  - Microinjections
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*physiology
MH  - Neurons, Afferent/*cytology
MH  - Proto-Oncogene Proteins/*physiology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptor Protein-Tyrosine Kinases/*physiology
MH  - Receptor, trkA
MH  - Receptors, Nerve Growth Factor/*physiology
PMC - PMC2290869
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
PMCR- 1994/06/02
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PHST- 1994/06/02 00:00 [pmc-release]
AID - 94075416 [pii]
AID - 10.1083/jcb.123.6.1555 [doi]
PST - ppublish
SO  - J Cell Biol. 1993 Dec;123(6 Pt 1):1555-66. doi: 10.1083/jcb.123.6.1555.