PMID- 8254194
OWN - NLM
STAT- MEDLINE
DCOM- 19940112
LR  - 20141120
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 152
IP  - 1
DP  - 1994 Jan 1
TI  - Production of IL-8 and monocyte chemotactic peptide-1 by peripheral blood 
      monocytes. Disparate responses to phytohemagglutinin and lipopolysaccharide.
PG  - 241-9
AB  - The temporal recruitment of leukocytes to a site of inflammation is dependent on 
      a complex interplay of a number of soluble mediators. Recently, two families of 
      chemotactic cytokines have been discovered. The -C-X-C-family, which includes 
      IL-8, appears to recruit neutrophils and lymphocytes. In contrast, the 
      -C-C-family, which includes monocyte chemotactic peptide-1 (MCP-1), appears to 
      recruit predominantly monocytes. Monocytes, after their arrival at a site of 
      inflammation, could further amplify the immune response by secreting IL-8 and 
      MCP-1. We sought to define conditions under which human peripheral blood 
      monocytes produce IL-8 and MCP-1. Using serum-free media, we found that 
      PHA-stimulated monocytes expressed MCP-1 and IL-8 protein and mRNA in a 
      dose-dependent manner. However, the onset of mRNA expression for MCP-1 occurred 
      at least 3 h later than did the onset of IL-8 mRNA expression. IL-8 and MCP-1 
      gene expression by monocytes appeared to require de novo protein synthesis, in 
      that cycloheximide blocked the expression of mRNA for both IL-8 and MCP-1 in 
      PHA-stimulated cells. However, treatment of monocytes with cycloheximide resulted 
      in the superinduction of IL-8 compared with control monocytes. Monocytes 
      costimulated with PHA and LPS demonstrated enhanced amounts of IL-8 mRNA and 
      protein, but sharply decreased amounts of MCP-1 mRNA and protein. The addition of 
      serum to culture media increased both the constitutive and PHA-induced production 
      of monocyte-derived MCP-1 and IL-8, but had no effect on the inhibition of 
      PHA-stimulated MCP-1 production by LPS. These findings suggest that distinct 
      pathways of activation exist for the production of monocyte-derived IL-8 and 
      MCP-1. The differential expression of these different but related polypeptides 
      may offer a means of control of the type of immune cells that are recruited to a 
      site of inflammation.
FAU - Liebler, J M
AU  - Liebler JM
AD  - Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
FAU - Kunkel, S L
AU  - Kunkel SL
FAU - Burdick, M D
AU  - Burdick MD
FAU - Standiford, T J
AU  - Standiford TJ
FAU - Rolfe, M W
AU  - Rolfe MW
FAU - Strieter, R M
AU  - Strieter RM
LA  - eng
GR  - 1P50HL46487/HL/NHLBI NIH HHS/United States
GR  - HL02401/HL/NHLBI NIH HHS/United States
GR  - HL31693/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Base Sequence
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/*biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - Monocytes/immunology/*metabolism
MH  - Phytohemagglutinins/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1994 Jan 1;152(1):241-9.