PMID- 8254466
OWN - NLM
STAT- MEDLINE
DCOM- 19940107
LR  - 20200220
IS  - 0894-9255 (Print)
IS  - 0894-9255 (Linking)
VI  - 6
IP  - 12
DP  - 1993 Dec
TI  - Fusion activity dissociated from replication ability in feline immunodeficiency 
      virus (FIV) in human cells.
PG  - 1301-10
AB  - Multinucleated-giant-cell formation followed by cell killing was observed after 
      cocultivation of the feline immunodeficiency virus (FIV)-producing feline T-cell 
      line 3201/FIV with various human cells, including T-cell lines carrying human 
      T-cell lymphotropic virus type I (HTLV-I). The susceptibility to giant cell 
      formation varied with the cell lines tested. Cocultivation of irradiated 3201/FIV 
      cells with MT-2 cells resulted in giant cell formation as early as 2 h in 
      culture, with striking resemblance to that induced by human immunodeficiency 
      virus (HIV). MT-4 cells (HTLV-I positive) and H9 cells (HTLV-I negative) were 
      less susceptible than MT-2 to the induction of syncytia. MOLT-4 cells (HTLV-I 
      negative) had intermediate sensitivity to syncytia formation. No syncytia were 
      observed in the monocytic cell line U-937 (HTLV-I negative). Syncytia formation 
      between 3201/FIV and MT-2 cells was inhibited by polyclonal cat anti-FIV antisera 
      but not polyclonal cat anti-feline leukemia virus (FeLV) antisera, goat 
      anti-FeLV, uninfected specific-pathogen-free cat serum, human anti-HTLV-I 
      antisera, or normal human and goat serum. Concentrated cell-free FIV supernatant 
      from 3201/FIV also induced giant cells of MT-2 cells that were indistinguishable 
      from those induced by cocultivation. Giant cells and extensive cell killing 
      associated with giant cell formation declined and disappeared within 10 days. 
      Surviving cells appeared to be of normal size and grew continuously without 
      expressing FIV antigen or releasing infective virus. Although Southern blot 
      analysis using probes specific for FIV could not detect proviral DNA in any of 
      the five human cell lines cocultured with irradiated 3201/FIV cells, the 
      polymerase chain reaction (PCR) technique detected FIV-specific DNA in MOLT-4 
      cells. DNA from the FIV-PCR positive MOLT-4 cells was PCR negative for endogenous 
      FeLV-specific sequences, indicating that the MOLT-4 cell DNA was not contaminated 
      with DNA from feline cells (i.e., 3201 cells). The FIV-MOLT-4 cells remained PCR 
      positive for FIV after 40 passages, suggesting stable integration in the human 
      cell line. These findings indicate that FIV is capable of forming proviral DNA in 
      human T-lymphoid cells by cocultivation, although this FIV-carrying human cell 
      line failed to produce replication-competent viruses.
FAU - Tochikura, T S
AU  - Tochikura TS
AD  - Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
FAU - Tanabe-Tochikura, A
AU  - Tanabe-Tochikura A
FAU - Hayes, K A
AU  - Hayes KA
FAU - Lazo, A
AU  - Lazo A
FAU - Bailer, R T
AU  - Bailer RT
FAU - Blakeslee, J R Jr
AU  - Blakeslee JR Jr
FAU - Lafrado, L J
AU  - Lafrado LJ
FAU - Roy-Burman, P
AU  - Roy-Burman P
FAU - Pandey, R
AU  - Pandey R
FAU - Olsen, R G
AU  - Olsen RG
AU  - et al.
LA  - eng
GR  - 5 P30 CA 16058/CA/NCI NIH HHS/United States
GR  - N01 AI-62525/AI/NIAID NIH HHS/United States
GR  - R01 CA-56295/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Acquir Immune Defic Syndr (1988)
JT  - Journal of acquired immune deficiency syndromes
JID - 8812597
RN  - 0 (DNA, Viral)
RN  - 0 (Immune Sera)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cats
MH  - Cell Fusion
MH  - Cell Line
MH  - Cell Survival
MH  - DNA, Viral/analysis
MH  - Fluorescent Antibody Technique
MH  - Giant Cells/cytology/*microbiology
MH  - Human T-lymphotropic virus 1
MH  - Humans
MH  - Immune Sera/immunology
MH  - Immunodeficiency Virus, Feline/genetics/*physiology
MH  - Polymerase Chain Reaction
MH  - Radioimmunoprecipitation Assay
MH  - Specific Pathogen-Free Organisms
MH  - Viral Proteins/biosynthesis
MH  - *Virus Replication
EDAT- 1993/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PST - ppublish
SO  - J Acquir Immune Defic Syndr (1988). 1993 Dec;6(12):1301-10.