PMID- 8254772
OWN - NLM
STAT- MEDLINE
DCOM- 19940113
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 68
IP  - 1
DP  - 1994 Jan
TI  - Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type 
      I basal gene expression.
PG  - 561-5
AB  - Sequences which control basal human T-cell lymphotropic virus type I (HTLV-I) 
      transcription likely play an important role in initiation and maintenance of 
      virus replication. We previously identified and analyzed a 45-nucleotide sequence 
      (downstream regulatory element 1 [DRE 1]), +195 to +240, at the boundary of the 
      R/U5 region of the long terminal repeat which is required for HTLV-I basal 
      transcription. We identified a protein, p37, which specifically bound to DRE 1. 
      An affinity column fraction, containing p37, stimulated HTLV-I transcription 
      approximately 12-fold in vitro. We now report the identification of a cDNA clone 
      (15B-7), from a Jurkat expression library, that binds specifically to the DRE 1 
      regulatory sequence. Binding of the cDNA fusion protein, similarly to the results 
      obtained with purified Jurkat protein, was decreased by introduction of 
      site-specific mutations in the DRE 1 regulatory sequence. In vitro transcription 
      and translation of 15B-7 cDNA produced a fusion protein which bound specifically 
      to the HTLV-I +195 to +240 oligonucleotide. The partial cDNA encodes a protein 
      which is homologous to the C-terminal 196 amino acids of the 36-kDa transcription 
      factor, YB-1. Cotransfection of a YB-1 expression plasmid increases HTLV-I basal 
      transcription approximately 14-fold in Jurkat T lymphocytes. On the basis of the 
      molecular weight, DNA-binding characteristics, and in vivo transactivation 
      activity, we suggest that the previously identified DRE 1-binding protein, p37, 
      is YB-1.
FAU - Kashanchi, F
AU  - Kashanchi F
AD  - Laboratory of Molecular Virology, National Institutes of Health, Bethesda, 
      Maryland 20892.
FAU - Duvall, J F
AU  - Duvall JF
FAU - Dittmer, J
AU  - Dittmer J
FAU - Mireskandari, A
AU  - Mireskandari A
FAU - Reid, R L
AU  - Reid RL
FAU - Gitlin, S D
AU  - Gitlin SD
FAU - Brady, J N
AU  - Brady JN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis
MH  - DNA-Binding Proteins/biosynthesis/*genetics
MH  - *Gene Expression Regulation, Viral
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - RNA, Messenger/analysis
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - T-Lymphocytes
MH  - Transcription Factors/biosynthesis/*genetics
MH  - Y-Box-Binding Protein 1
PMC - PMC236322
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
PMCR- 1994/01/01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PHST- 1994/01/01 00:00 [pmc-release]
AID - 10.1128/JVI.68.1.561-565.1994 [doi]
PST - ppublish
SO  - J Virol. 1994 Jan;68(1):561-5. doi: 10.1128/JVI.68.1.561-565.1994.