PMID- 8258392
OWN - NLM
STAT- MEDLINE
DCOM- 19940119
LR  - 20190909
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 21
IP  - 3
DP  - 1993 Oct
TI  - Identification of stage-specific changes in protein secretion by isolated 
      seminiferous tubules from the rat following exposure to either m-dinitrobenzene 
      or nitrobenzene.
PG  - 384-92
AB  - The objective of this study was to identify effects of two known Sertoli cell 
      toxicants on the secretion of proteins by seminiferous tubules (ST) isolated from 
      adult rats at different stages of the spermatogenic cycle and cultured in vitro 
      for 24 hr with [35S]methionine. Adult rats received a single oral dose of 50 
      mg/kg metadinitrobenzene (m-DNB) or 300 mg/kg nitrobenzene (NB). Long lengths of 
      ST at stages II-V, VI-VIII or IX-XII were then isolated from control and treated 
      rats at 1 or 3 days post-treatment; selection of stages was based on the stage 
      specificity of the early (24-72 hr) adverse effects of m-DNB and NB on 
      spermatogenesis in vivo. In addition, ST at the same stages were isolated from 
      untreated rats and cultured in the presence or absence of m-DNB or NB (10(-4)M). 
      Incorporation of [35S]methionine into secreted proteins was assessed and the 
      pattern of protein secretion evaluated using two-dimensional sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis (2-D SDS-PAGE). ST isolated from rats 
      pretreated 24 hr earlier with NB in vivo showed a significant decrease in the 
      overall incorporation of [35S]methionine into secreted proteins at stages VI-VIII 
      and IX-XII, whereas ST at stages II-V showed no such change; comparable protein 
      changes were observed when 10(-4) M NB was added in vitro for 24 hr to ST 
      isolated at the same stages from untreated rats.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - McLaren, T T
AU  - McLaren TT
AD  - MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, 
      Scotland.
FAU - Foster, P M
AU  - Foster PM
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Proteins)
RN  - 0 (Sulfur Radioisotopes)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - AE28F7PNPL (Methionine)
RN  - DK8B627BU0 (3-dinitrobenzene)
RN  - E57JCN6SSY (nitrobenzene)
SB  - IM
MH  - Animals
MH  - Dinitrobenzenes/*toxicity
MH  - Electrophoresis, Polyacrylamide Gel
MH  - In Vitro Techniques
MH  - Male
MH  - Methionine/metabolism
MH  - Nitrobenzenes/*toxicity
MH  - Organ Size/drug effects
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Seminiferous Tubules/*drug effects
MH  - Sodium Dodecyl Sulfate
MH  - Spermatogenesis/drug effects/*physiology
MH  - Sulfur Radioisotopes
MH  - Testis/anatomy & histology/drug effects
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - S0272059083711115 [pii]
AID - 10.1006/faat.1993.1111 [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1993 Oct;21(3):384-92. doi: 10.1006/faat.1993.1111.