PMID- 8258721
OWN - NLM
STAT- MEDLINE
DCOM- 19940114
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 151
IP  - 12
DP  - 1993 Dec 15
TI  - Recruitment of inflammatory cells to the pleural space. Chemotactic cytokines, 
      IL-8, and monocyte chemotactic peptide-1 in human pleural fluids.
PG  - 7216-23
AB  - Pleural effusions secondary to various diseases are associated with the presence 
      of different inflammatory cells. The role of selective chemotactic cytokines in 
      the recruitment of phagocytes to the pleural space is unclear. IL-8 and monocyte 
      chemotactic peptide-1 (MCP-1) are recently described cytokines that are 
      chemotactic for neutrophils and monocytes, respectively. We prospectively studied 
      63 patients, using strictly defined criteria for their selection. IL-8 
      concentrations were elevated in both empyema fluid (9.15 +/- 0.89 ng/ml) and 
      parapneumonic effusions (4.7 +/- 0.697 ng/ml) when compared with pleural 
      effusions secondary to other diseases. IL-8 levels were higher in empyema fluid 
      than in parapneumonic effusions (p = 0.01). There was a significant correlation 
      between IL-8 levels and the total numbers of neutrophils in empyema fluids (r = 
      0.80). Chemotactic activity for neutrophils was elevated in empyema fluid and the 
      addition of IL-8 neutralizing serum decreased bioactivity by 32.22%. Malignant 
      pleural effusions had the highest levels of MCP-1 (12.0 +/- 3.7 ng/ml) when 
      compared with others. Cytology-positive pleural fluids (n = 10) had a higher 
      level of MCP-1 than cytology-negative effusions (p = < 0.05). Malignant pleural 
      fluid MCP-1 levels correlated (r = 0.70) with the absolute number of monocytes in 
      the pleural fluid. Neutralization of monocyte chemotactic activity of malignant 
      pleural fluid by specific neutralizing serum caused a 70.3% inhibition of 
      bioactivity. Immunohistochemical staining of malignant pleural fluid localized 
      antigenic MCP-1 to malignant cells. We conclude that both IL-8 and MCP-1 play 
      major but not exclusive roles in the recruitment of neutrophils and monocytes 
      from the vascular compartment to the pleural space.
FAU - Antony, V B
AU  - Antony VB
AD  - Department of Pulmonary and Critical Care Medicine, Veterans Administration 
      Medical Center, Indianapolis, Indiana.
FAU - Godbey, S W
AU  - Godbey SW
FAU - Kunkel, S L
AU  - Kunkel SL
FAU - Hott, J W
AU  - Hott JW
FAU - Hartman, D L
AU  - Hartman DL
FAU - Burdick, M D
AU  - Burdick MD
FAU - Strieter, R M
AU  - Strieter RM
LA  - eng
GR  - R01 AA08285/AA/NIAAA NIH HHS/United States
GR  - R01 HL44281/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*metabolism
MH  - Cytokines/*metabolism
MH  - Empyema/immunology/pathology
MH  - Heart Failure/immunology/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-8/*metabolism
MH  - Pleural Effusion/*immunology/*pathology
MH  - Pleural Effusion, Malignant/immunology/pathology
MH  - Pleurisy/etiology/immunology/pathology
MH  - Pneumonia/immunology/pathology
MH  - Tuberculosis, Pulmonary/immunology/pathology
EDAT- 1993/12/15 00:00
MHDA- 1993/12/15 00:01
CRDT- 1993/12/15 00:00
PHST- 1993/12/15 00:00 [pubmed]
PHST- 1993/12/15 00:01 [medline]
PHST- 1993/12/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1993 Dec 15;151(12):7216-23.