PMID- 8260719
OWN - NLM
STAT- MEDLINE
DCOM- 19940127
LR  - 20061115
IS  - 0916-8478 (Print)
IS  - 0916-8478 (Linking)
VI  - 38
IP  - 3
DP  - 1993 Sep
TI  - Cosmid clones from microdissected human chromosomal region 15q11-q13.
PG  - 267-75
AB  - A human chromosomal region, 15q11-q13, was microdissected, its DNA was amplified 
      with the primer-linker PCR method, and the PCR products were cloned into a 
      plasmid vector to construct a microclone library. Of 193 microclones analyzed 
      with Southern blot hybridization on hybrid cell panels, 26 (13.5%) were either 
      single-copy (unique) or low-repetitive fragments. By screening of a cosmid 
      library of human genomic DNA using the 26 microclones as probes, 47 positive 
      cosmids were obtained and underwent regional mapping with chromosome fluorescence 
      in situ hybridization (FISH). Sixteen cosmids gave FISH signals at 15p-cen, 5 at 
      15q11-q13, 6 at 15q22-q26, 3 at other chromosomes, and 17 no signal. These 27 
      cosmids mapped to chromosome 15 are useful additions to the inventory of DNA 
      markers of this chromosome including the much interested Prader-Willi/Angelman 
      syndrome region.
FAU - Tohma, T
AU  - Tohma T
AD  - Department of Pediatrics, University of the Ryukyus School of Medicine, Okinawa, 
      Japan.
FAU - Tamura, T
AU  - Tamura T
FAU - Ohta, T
AU  - Ohta T
FAU - Soejima, H
AU  - Soejima H
FAU - Kubota, T
AU  - Kubota T
FAU - Jinno, Y
AU  - Jinno Y
FAU - Tsukamoto, K
AU  - Tsukamoto K
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Naritomi, K
AU  - Naritomi K
FAU - Niikawa, N
AU  - Niikawa N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Hum Genet
JT  - The Japanese journal of human genetics
JID - 9213239
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 15
MH  - Cloning, Molecular
MH  - Cosmids/*genetics
MH  - *DNA
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1007/BF01874137 [doi]
PST - ppublish
SO  - Jpn J Hum Genet. 1993 Sep;38(3):267-75. doi: 10.1007/BF01874137.