PMID- 8261020
OWN - NLM
STAT- MEDLINE
DCOM- 19940125
LR  - 20191101
IS  - 1016-0922 (Print)
IS  - 1016-0922 (Linking)
VI  - 2
IP  - 2
DP  - 1993 Mar-Apr
TI  - Protein kinase C as a multi-targeted feedback inhibitor regulating the Ca2+ 
      responses to chemotactic peptide stimulation in the murine macrophage cell line 
      PU5-1.8.
PG  - 84-94
AB  - The role of protein kinase C (PKC) in the signal transduction process when 
      macrophage cells were stimulated by the chemotactic peptide 
      formyl-methionyl-leucyl-phenylalanine (fMLP) was investigated in the cell line 
      PU5-1.8. Addition of fMLP to PU5-1.8 cells induced a rapid generation of inositol 
      trisphosphate and a translocation of PKC. Concomitantly there was an influx of 
      Ca2+ followed by its expulsion. PKC was likely involved in Ca2+ extrusion as 
      short-term pretreatment of cells with phorbol 12-myristate 13-acetate (PMA) 
      enhanced the removal of preloaded 45Ca2+ but had no significant effect on 
      fMLP-stimulated 45Ca2+ influx. Depletion of cellular PKC activity through 
      down-regulation or exposure of cells to PKC inhibitors also blocked the 
      fMLP-stimulated 45Ca2+ efflux. Further experiments using PMA revealed that PKC 
      also decreased vesicular Ca2+ and stimulated the catabolism of inositol 
      1,4,5-trisphosphate. These results lend support to the notion that PKC serves as 
      a negative feedback modulator in Ca2+ signalling and further suggest that it 
      operates at several target sites.
FAU - Kong, S K
AU  - Kong SK
AD  - Department of Biochemistry, Chinese University of Hong Kong, Shatin, NT.
FAU - Choy, Y M
AU  - Choy YM
FAU - Lee, C Y
AU  - Lee CY
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Biol Signals
JT  - Biological signals
JID - 9210083
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Feedback
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Protein Kinase C/*physiology
MH  - Signal Transduction
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
AID - 10.1159/000109480 [doi]
PST - ppublish
SO  - Biol Signals. 1993 Mar-Apr;2(2):84-94. doi: 10.1159/000109480.