PMID- 8262382
OWN - NLM
STAT- MEDLINE
DCOM- 19940125
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 134
IP  - 2
DP  - 1993 Dec 8
TI  - Cloning of a cDNA encoding a group-V (group-IX) allergen isoform from rye-grass 
      pollen that demonstrates specific antigenic immunoreactivity.
PG  - 235-40
AB  - We have isolated and characterized the cDNA clone, 19R, that encodes an isoform 
      of a major rye-grass pollen allergen, Lol p V [previously referred to as Lol p 
      1b; Singh et al., Proc. Natl. Acad. Sci. USA 88 (1991) 1384-1388; and Lol p IX; 
      Suphioglu et al., Lancet 339 (1992) 569-572]. Clone 19R was isolated from a 
      rye-grass pollen cDNA expression library using grass pollen-specific 
      immunoglobulin E (IgE) antibodies (Ab) from an allergic serum pool. The 
      nucleotide (nt) sequence of clone 19R potentially encodes a 33.8-kDa protein of 
      339 amino acids (aa). It possesses a leader peptide essentially identical to the 
      previously characterized isoform of Lol p V (Lol p VA). This indicates a mature 
      processed 31.3-kDa protein of 314 aa, correlating well with the size of the 
      polypeptides revealed by Western analysis of pollen proteins using IgE Ab 
      affinity purified from recombinant fusion protein (reFP) encoded by clone 19R as 
      solid matrix. There is no N-glycosylation motif. The protein encoded by clone 
      19R, designated Lol p VB, has 66.4% identity and 80.4% similarity with Lol p VA. 
      However, a Lol p VA-specific monoclonal Ab, FMC A7, does not recognize reFP 
      encoded by clone 19R, indicating that Lol p VB does not share this epitope. 
      Cross-reactivity studies using affinity purified IgE Ab showed that both isoforms 
      share similar allergenic epitopes. Immunoblot analysis using sera from a 
      population of 30 patients showed that 80% possess IgE Ab that recognize both Lol 
      p V isoforms. Variation occurred in the signal intensities of IgE 
      binding.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Ong, E K
AU  - Ong EK
AD  - School of Botany, University of Melbourne, Parkville, Vic., Australia.
FAU - Griffith, I J
AU  - Griffith IJ
FAU - Knox, R B
AU  - Knox RB
FAU - Singh, M B
AU  - Singh MB
LA  - eng
SI  - GENBANK/L13083
SI  - GENBANK/L19066
SI  - GENBANK/L24529
SI  - GENBANK/Z14103
SI  - GENBANK/Z14104
SI  - GENBANK/Z14105
SI  - GENBANK/Z14106
SI  - GENBANK/Z14107
SI  - GENBANK/Z15047
SI  - GENBANK/Z15048
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Lol p V protein, Lolium perenne)
RN  - 0 (Plant Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Allergens/*genetics/immunology
MH  - Antigens, Plant
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - Cross Reactions
MH  - DNA
MH  - Lolium/*genetics
MH  - Molecular Sequence Data
MH  - Plant Proteins/*genetics/immunology
MH  - Pollen/*genetics/immunology
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1993/12/08 00:00
MHDA- 1993/12/08 00:01
CRDT- 1993/12/08 00:00
PHST- 1993/12/08 00:00 [pubmed]
PHST- 1993/12/08 00:01 [medline]
PHST- 1993/12/08 00:00 [entrez]
AID - 0378-1119(93)90099-O [pii]
AID - 10.1016/0378-1119(93)90099-o [doi]
PST - ppublish
SO  - Gene. 1993 Dec 8;134(2):235-40. doi: 10.1016/0378-1119(93)90099-o.