PMID- 8262546
OWN - NLM
STAT- MEDLINE
DCOM- 19940124
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 80
IP  - 2
DP  - 1993 Oct
TI  - Clonal variations in complement activation and deposition of C3b and C4b on model 
      immune complexes.
PG  - 168-76
AB  - This study examined the relationship between complement activation and the 
      deposition of C3b and C4b on a panel of model immune complexes (IC). IC were 
      constructed by combining murine monoclonal IgM, IgA, IgG1, IgG2a or IgG3 
      anti-dinitrophenyl (DNP) antibodies with DNP-bovine serum albumin (DNP-BSA). The 
      IC were incubated with human plasma as a complement source and the formation of 
      C4a and C3a, as well as the deposition of C4b and C3b on the IC, measured by 
      radioimmunoassay. The results indicate that there were isotype-independent 
      variations in the capacity of different types of IC to activate the classical 
      pathway, especially for isotype-matched pairs of IC containing IgG1, IgG2a and 
      IgG3 antibodies. In most cases, there was a direct relationship between classical 
      pathway activation and the cleavage of C3. There was, for most of the IC, a 
      direct correlation between cleavage of C4 and C3 and the subsequent deposition of 
      C4b and C3b on the IC. However, a pair of IC constructed with independently 
      derived IgG1 antibodies was virtually identical with respect to C3 cleavage and 
      yet differed in the number of C3b molecules deposited on the IC. Collectively, 
      these data suggest that the immunoglobulin variable region can play a significant 
      role in both complement activation and the deposition of C3b and C4b on IC.
FAU - Yokoyama, I
AU  - Yokoyama I
AD  - Department of Microbiology and Immunology, University of Oklahoma Health Sciences 
      Center, Oklahoma City 73190.
FAU - Waxman, F
AU  - Waxman F
LA  - eng
GR  - R01 AI 25964/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Dinitrophenols)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (dinitrophenyl-bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 80295-43-8 (Complement C3b)
RN  - 80295-50-7 (Complement C4b)
SB  - IM
MH  - Antigen-Antibody Complex/*immunology
MH  - Complement Activation/*immunology
MH  - Complement C3b/*immunology
MH  - Complement C4b/*immunology
MH  - Dinitrophenols/immunology
MH  - Humans
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin G/immunology
MH  - Immunoglobulin M/immunology
MH  - Kinetics
MH  - Serum Albumin, Bovine/immunology
PMC - PMC1422188
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
PMCR- 1994/10/01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1993 Oct;80(2):168-76.