PMID- 8262620
OWN - NLM
STAT- MEDLINE
DCOM- 19940127
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 62
IP  - 1
DP  - 1994 Jan
TI  - Effect of treatment on titer, function, and antigen recognition of serum 
      antibodies to Actinobacillus actinomycetemcomitans in patients with rapidly 
      progressive periodontitis.
PG  - 145-51
AB  - Although periodontal treatment by scaling and root planing (SCRP) is known to 
      induce bacteremia, the effect of this procedure on the host immune response is 
      not known. We have determined pre- and post-SCRP immunoglobulin G antibody titers 
      to antigens of Actinobacillus actinomycetemcomitans in the sera of 22 patients 
      with rapidly progressive periodontitis. We also assessed the ability of these 
      sera to enhance phagocytosis and killing of A. actinomycetemcomitans by human 
      polymorphonuclear leukocytes by using a polymorphonuclear leukocyte 
      chemiluminescence (CL) assay. Specific anti-A. actinomycetemcomitans antibody 
      titers were significantly increased at 6 and 12 months after beginning treatment, 
      and CL values were significantly increased at 12 months, whereas mean 
      interproximal pocket depths were significantly decreased at 12 months after 
      beginning treatment. When patients were classified as either seropositive (twice 
      the median titer of control subjects; n = 10) or seronegative (n = 12), both 
      median titers and CL values were significantly increased for the seronegative 
      group at 6 and 12 months after treatment. In the seropositive group, only the 
      median titer was significantly increased at 12 months. Western blot (immunoblot) 
      patterns for six seronegative and six seropositive patients differed remarkably 
      at the baseline. Before treatment, all of the seropositive patients recognized 
      high-molecular-mass lipopolysaccharide (LPS) and a large number of protein 
      components. Patterns were virtually unaffected by therapy. Before treatment, only 
      one of the seronegative patients recognized the LPS smear and none reacted 
      strongly with protein components. Following treatment, slight LPS staining was 
      observed for five of six seronegative patients and detection of protein bands was 
      enhanced in all cases. We conclude that treatment by SCRP induces a humoral 
      immune response, especially in seronegative patients, and that response may play 
      a role in the observed beneficial effects of periodontal treatment.
FAU - Sjostrom, K
AU  - Sjostrom K
AD  - Department of Periodontics, School of Dentistry, University of Washington, 
      Seattle 98195.
FAU - Ou, J
AU  - Ou J
FAU - Whitney, C
AU  - Whitney C
FAU - Johnson, B
AU  - Johnson B
FAU - Darveau, R
AU  - Darveau R
FAU - Engel, D
AU  - Engel D
FAU - Page, R C
AU  - Page RC
LA  - eng
GR  - 1 P01 DE08555/DE/NIDCR NIH HHS/United States
GR  - T32 DE07063/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Opsonin Proteins)
SB  - IM
MH  - Adult
MH  - Aggregatibacter actinomycetemcomitans/*immunology
MH  - Antibodies, Bacterial/*immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Opsonin Proteins
MH  - Periodontitis/*immunology/therapy
MH  - Time Factors
PMC - PMC186079
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
PMCR- 1994/01/01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PHST- 1994/01/01 00:00 [pmc-release]
AID - 10.1128/iai.62.1.145-151.1994 [doi]
PST - ppublish
SO  - Infect Immun. 1994 Jan;62(1):145-51. doi: 10.1128/iai.62.1.145-151.1994.