PMID- 8263009
OWN - NLM
STAT- MEDLINE
DCOM- 19940127
LR  - 20190904
IS  - 0171-5216 (Print)
IS  - 0171-5216 (Linking)
VI  - 120
IP  - 3
DP  - 1994
TI  - Gene analysis of K-, H-ras, p53, and retinoblastoma susceptibility genes in human 
      lung cancer cell lines by the polymerase chain reaction/single-strand 
      conformation polymorphism method.
PG  - 143-8
AB  - In order to know the involvement of multiple gene alterations in the pathogenesis 
      of human lung cancer, we examined the genes of K-, H-ras (codons 12, 13, 61), 
      p53(exons 5-9) and the retinoblastoma susceptibility gene (RB)(exons 20-22) using 
      the polymerase chain reaction/single-strand conformation polymorphism method in 
      32 human lung cancer cell lines (5 squamous-cell carcinomas, 10 adenocarcinomas, 
      3 large-cell carcinomas, 14 small-cell carcinomas). In 18 non-small-cell lung 
      cancer lines, gene alterations were found in 4 for K-ras (22%), none for H-ras 
      (0%), 4 for p53 (22%) and none for the RB (0%) gene. In 14 small-cell lung cancer 
      (SCLC) lines, no gene alterations were found in K-ras (0%), or H-ras (0%), but 6 
      were found for p53 (43%) and 3 for the RB (21%) gene. Coincident abnormalities of 
      K-ras and p53, or K-ras and RB genes were not found in any cell lines, and those 
      of the p53 and RB genes were found in only 2 SCLC lines. No association was 
      observed between these three gene alterations and N-myc amplification. Although 
      the above three genes may be involved to some extent in the pathogenesis of lung 
      cancer, more factors are required for its development.
FAU - Kashii, T
AU  - Kashii T
AD  - First Department of Internal Medicine, Toyama Medical and Pharmaceutical 
      University, Japan.
FAU - Mizushima, Y
AU  - Mizushima Y
FAU - Monno, S
AU  - Monno S
FAU - Nakagawa, K
AU  - Nakagawa K
FAU - Kobayashi, M
AU  - Kobayashi M
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
SB  - IM
GS  - H-ras
GS  - K-ras
GS  - N-myc
GS  - p53
MH  - Aged
MH  - Base Sequence
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - Carcinoma, Small Cell/*genetics
MH  - Female
MH  - Genes, Retinoblastoma/*genetics
MH  - Genes, myc/genetics
MH  - Genes, p53/*genetics
MH  - Genes, ras/*genetics
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Point Mutation/*genetics
MH  - Polymerase Chain Reaction/methods
MH  - Sequence Analysis, DNA
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF01202192 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 1994;120(3):143-8. doi: 10.1007/BF01202192.