PMID- 8263030
OWN - NLM
STAT- MEDLINE
DCOM- 19940126
LR  - 20151119
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 158
IP  - 1
DP  - 1994 Jan
TI  - Disparate modulation of plasma membrane protein lateral mobility by various cell 
      permeabilizing agents.
PG  - 7-16
AB  - The mobility of a cell surface protein on cells osmotically swollen by treatment 
      with several different cell permeabilizing agents retains specific restraints 
      despite detachment of the plasma membrane from the cortical cytoskeleton. 
      Fluorescence photobleaching recovery experiments indicate that the lateral 
      diffusion constants of immunoglobulin E (IgE)-receptor complexes on the surface 
      of rat basophilic leukemia cells increase 2-5x following permeabilization with 
      streptolysin O or digitonin, with little change in their mobile fractions. 
      Swelling by hypo-osmotic treatment in water enhances lateral diffusion of 
      IgE-receptor complexes and raises the mobile fractions to near 100%. In contrast, 
      swelling by treatment with filipin arrests lateral diffusion, although rotational 
      mobility remains unhindered. Lateral mobility of a fluorescent lipid analogue 
      remains unchanged under these conditions. Crosslinking by anti-IgE antibodies 
      redistributes the IgE-receptor complexes into large patches on untreated cells 
      and on cells swollen by permeabilization with streptolysin O or digitonin, but 
      not on cells swollen by treatment with filipin. The results indicate a diversity 
      of effects of the various permeabilizing agents on the mobility of membrane 
      proteins. In particular, treatment with filipin appears to reorganize the plasma 
      membrane into a network of fluid domains on a scale smaller than the bleaching 
      spot size used (approximately 1.5 microns).
FAU - Feder, T J
AU  - Feder TJ
AD  - Department of Physics, Cornell University, Ithaca, New York 14853.
FAU - Chang, E Y
AU  - Chang EY
FAU - Holowka, D
AU  - Holowka D
FAU - Webb, W W
AU  - Webb WW
LA  - eng
GR  - 08-PIRRO4224A/RR/NCRR NIH HHS/United States
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI22449/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Bacterial Proteins)
RN  - 0 (Fluoresceins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Streptolysins)
RN  - 0 (streptolysin O)
RN  - 059QF0KO0R (Water)
RN  - 87Z59R7D14 (Filipin)
RN  - KOO5CM684H (Digitonin)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins
MH  - Biological Transport/drug effects
MH  - Cell Membrane Permeability
MH  - Diffusion
MH  - Digitonin/*pharmacology
MH  - Filipin/*pharmacology
MH  - Fluorescein
MH  - Fluoresceins
MH  - Membrane Proteins/*metabolism
MH  - Microscopy, Fluorescence/methods
MH  - Osmosis
MH  - Rats
MH  - Receptors, IgE/metabolism
MH  - Streptolysins/*pharmacology
MH  - Tumor Cells, Cultured
MH  - Water/metabolism
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/jcp.1041580103 [doi]
PST - ppublish
SO  - J Cell Physiol. 1994 Jan;158(1):7-16. doi: 10.1002/jcp.1041580103.