PMID- 8264613
OWN - NLM
STAT- MEDLINE
DCOM- 19940121
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 14
IP  - 1
DP  - 1994 Jan
TI  - In vitro selection of DNA elements highly responsive to the human T-cell 
      lymphotropic virus type I transcriptional activator, Tax.
PG  - 456-62
AB  - The human T-cell lymphotropic virus type I (HTLV-I) transactivator, Tax, the 
      ubiquitous transcriptional factor cyclic AMP (cAMP) response element-binding 
      protein (CREB protein), and the 21-bp repeats in the HTLV-I transcriptional 
      enhancer form a ternary nucleoprotein complex (L. J. Zhao and C. Z. Giam, Proc. 
      Natl. Acad. Sci. USA 89:7070-7074, 1992). Using an antibody directed against the 
      COOH-terminal region of Tax along with purified Tax and CREB proteins, we 
      selected DNA elements bound specifically by the Tax-CREB complex in vitro. Two 
      distinct but related groups of sequences containing the cAMP response element 
      (CRE) flanked by long runs of G and C residues in the 5' and 3' regions, 
      respectively, were preferentially recognized by Tax-CREB. In contrast, CREB alone 
      binds only to CRE motifs (GNTGACG[T/C]) without neighboring G- or C-rich 
      sequences. The Tax-CREB-selected sequences bear a striking resemblance to the 5' 
      or 3' two-thirds of the HTLV-I 21-bp repeats and are highly inducible by Tax. Gel 
      electrophoretic mobility shift assays, DNA transfection, and DNase I footprinting 
      analyses indicated that the G- and C-rich sequences flanking the CRE motif are 
      crucial for Tax-CREB-DNA ternary complex assembly and Tax transactivation but are 
      not in direct contact with the Tax-CREB complex. These data show that Tax 
      recruits CREB to form a multiprotein complex that specifically recognizes the 
      viral 21-bp repeats. The expanded DNA binding specificity of Tax-CREB and the 
      obligatory role the ternary Tax-CREB-DNA complex plays in transactivation reveal 
      a novel mechanism for regulating the transcriptional activity of leucine zipper 
      proteins like CREB.
FAU - Paca-Uccaralertkun, S
AU  - Paca-Uccaralertkun S
AD  - Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
FAU - Zhao, L J
AU  - Zhao LJ
FAU - Adya, N
AU  - Adya N
FAU - Cross, J V
AU  - Cross JV
FAU - Cullen, B R
AU  - Cullen BR
FAU - Boros, I M
AU  - Boros IM
FAU - Giam, C Z
AU  - Giam CZ
LA  - eng
GR  - CA48709/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
SB  - IM
GS  - Tax
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cyclic AMP Response Element-Binding Protein/genetics/metabolism
MH  - DNA, Viral/*genetics/*metabolism
MH  - Gene Products, tax/*metabolism
MH  - Genes, pX
MH  - Human T-lymphotropic virus 1/*genetics/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Leucine Zippers/genetics
MH  - Molecular Sequence Data
MH  - Transcription, Genetic
MH  - Transcriptional Activation
PMC - PMC358395
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
PMCR- 1994/01/01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PHST- 1994/01/01 00:00 [pmc-release]
AID - 10.1128/mcb.14.1.456-462.1994 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1994 Jan;14(1):456-62. doi: 10.1128/mcb.14.1.456-462.1994.