PMID- 8267393
OWN - NLM
STAT- MEDLINE
DCOM- 19940121
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 13
IP  - 5C
DP  - 1993 Sep-Oct
TI  - Chromosomal heterogeneity of aneuploid leukemic cell populations detected by 
      conventional karyotyping and by fluorescence in situ hybridization (FISH).
PG  - 1857-62
AB  - Beside the frequent aneusomies of chromosomes # 7 and # 8 gains or losses of 
      several other chromosomes are found in bone marrow cells of leukemia patients. 
      Chromosomal heterogeneity of interphase cell populations was studied by 
      fluorescence in situ hybridization (FISH) with centromeric DNA probes for 
      chromosomes #2, #3, #4, #6, #9, #11, #12, #15, #16, #17, #18, #20, as well as X 
      and Y which were found to be aberrant by routine karyotyping of 28 cases of 
      various malignant hematopoietic diseases. Particularly, the data obtained by both 
      routes of analysis were compared quantitatively. As the most prominent result, 
      all aberrations found by classical karyotyping were redetected by interphase 
      cytogenetics, but additional aberrant clones could be observed among the 
      interphase cell populations. The frequencies of the cell clones with hypersomies 
      were in general higher in metaphase than in interphase, and, vice versa, 
      monosomic cells were found more frequently in interphase than in metaphase. 
      Single aberrant karyotypes in all cases were redetected as microclones of 
      interphase cells. Interphase cytogenetics using FISH, therefore, was shown not 
      only to be a reliable measure of the genomic heterogeneity of leukemic cell 
      populations but, in addition, to be a valuable and informative supplement to 
      routine leukemia cytogenetics with regard to the detection of microclones which, 
      later on, could dominate the progression of the malignant disease.
FAU - Gebhart, E
AU  - Gebhart E
AD  - Institut fur Humangenetik, Universitat Erlangen-Nurnberg, Germany.
FAU - Trautmann, U
AU  - Trautmann U
FAU - Reichardt, S
AU  - Reichardt S
FAU - Liehr, T
AU  - Liehr T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - Chromosome Aberrations/*diagnosis
MH  - Chromosome Disorders
MH  - DNA, Satellite
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Leukemia/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Mosaicism
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1993 Sep-Oct;13(5C):1857-62.