PMID- 8270247
OWN - NLM
STAT- MEDLINE
DCOM- 19940203
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 93
IP  - 1
DP  - 1994 Jan
TI  - Ordering markers in the region of the ataxia-telangiectasia gene (11q22-q23) by 
      fluorescence in situ hybridization (FISH) to interphase nuclei.
PG  - 1-6
AB  - Fluorescence in situ hybridization (FISH) to interphase nuclei was performed to 
      order probes corresponding to bands 11q22-q23 where the ataxia-telangiectasia 
      (AT) gene(s) have been located. Cosmid probes and one phage probe previously 
      localized to this chromosome 11 region by FISH to metaphase chromosomes, were 
      hybridized to interphase nuclei of the somatic cell hybrid J1a, which contains 
      chromosome 11 as the only human chromosome. Two-color FISH was used with a 
      centromeric reference probe marker. The following order was obtained: cen-D11S385 
      (CJ52.75)-CJ52.3-D11S384 (CJ52.193)-CJ52.114-D11S424 
      (CJ52.77)-D11S132-NCAM-D11S351 (CJ52.208)-tel. The validity of using the 
      centromeric probe was illustrated by showing that a probe corresponding to 11p13 
      hybridized more closely to the centromere than a probe corresponding to 
      11q22-q23, and by using cosmids hybridized three by three.
FAU - Cherif, D
AU  - Cherif D
AD  - Unite INSERM U 301, Paris, France.
FAU - Der-Sarkissian, H
AU  - Der-Sarkissian H
FAU - Berger, R
AU  - Berger R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Ataxia Telangiectasia/*genetics
MH  - Cell Nucleus
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - Cosmids
MH  - DNA Probes
MH  - Fluorescent Dyes
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Male
MH  - Metaphase/genetics
MH  - Polymerase Chain Reaction
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF00218903 [doi]
PST - ppublish
SO  - Hum Genet. 1994 Jan;93(1):1-6. doi: 10.1007/BF00218903.