PMID- 8275935
OWN - NLM
STAT- MEDLINE
DCOM- 19940207
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 134
IP  - 1
DP  - 1994 Jan
TI  - Expression of tumor necrosis factor-alpha (TNF alpha) receptors and selective 
      effect of TNF alpha on the inner cell mass in mouse blastocysts.
PG  - 206-12
AB  - The presence of tumor necrosis factor-alpha (TNF alpha) receptors was 
      demonstrated at the cell surface of mouse blastocysts by indirect 
      immunofluorescence. Amplification of total cDNA from these embryos indicated that 
      only the p60 form of the TNF alpha receptor was expressed. Amplification of the 
      p80 form remained negative. Blastocysts were cultured with 0.5, 5.0, or 50 ng/ml 
      TNF alpha and examined for their morphology and cell proliferation rate. Doses of 
      5.0 and 50 ng/ml delayed the morphological development after 24 h, but this 
      effect was no longer detected after 48 h. Overall cell proliferation was 
      decreased by 15% with 50 ng/ml TNF alpha after 24 h and with all three 
      concentrations after 48 h. Differential staining of the two embryonic cell 
      lineages revealed that the reduction in cell number was at the expense of the 
      inner cell mass, the cell group responsible for forming the fetal layers after 
      implantation. This inhibition was not mediated by cytotoxicity, as the proportion 
      of dead ICM cells remained low in the presence of TNF alpha. Our data indicate 
      that the expression of TNF alpha receptors is developmentally regulated before 
      implantation, and that preimplantation embryos are responsive to TNF alpha.
FAU - Pampfer, S
AU  - Pampfer S
AD  - Physiology of Human Reproduction Research Unit, University of Louvain School of 
      Medicine, Brussels, Belgium.
FAU - Wuu, Y D
AU  - Wuu YD
FAU - Vanderheyden, I
AU  - Vanderheyden I
FAU - De Hertogh, R
AU  - De Hertogh R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blastocyst/cytology/*metabolism
MH  - Cell Division
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Cell Survival
MH  - Fluorescent Antibody Technique
MH  - Mice
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1210/endo.134.1.8275935 [doi]
PST - ppublish
SO  - Endocrinology. 1994 Jan;134(1):206-12. doi: 10.1210/endo.134.1.8275935.