PMID- 8277222
OWN - NLM
STAT- MEDLINE
DCOM- 19940210
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 138
IP  - 3
DP  - 1993 Sep
TI  - Protein kinase in rat adipocytes: influence of androgenic status and regional fat 
      distribution.
PG  - 493-501
AB  - The effects of castration and testosterone treatment on insulin- and phorbol 
      ester (TPA)-stimulated lipogenic responses, phorbol dibutyrate-specific binding 
      to protein kinase C (PKC) in the cytosol and the beta-PKC isoform level 
      quantified by immunoblotting were compared in rat fat cells from femoral 
      subcutaneous (SC) and deep intra-abdominal (epididymal) fat deposits. In control 
      rats, the PKC content was lower in SC than in epididymal fat cells. After 
      castration, the difference in PKC content between SC and epididymal fat cells was 
      reduced and restored by testosterone treatment. However, androgenic status failed 
      to modify the PKC content in SC fat cells. The lipogenic response to insulin was 
      also differently regulated by the androgenic status in the two fat deposits. 
      After castration, the response was increased in SC fat cells, while it was 
      blunted in epididymal fat cells. These effects were corrected by testosterone 
      administration. These results demonstrate that, in white adipocytes, PKC is an 
      additional biological parameter which varies according to the anatomical origin 
      of the fat cells. They also provide evidence that PKC is controlled by androgens 
      in vivo and emphasize the regional site specificity of such a control.
FAU - Lacasa, D
AU  - Lacasa D
AD  - Department of Biochemistry, Faculty of Medicine, Paris-Ouest, Centre Hospitalier 
      de Poissy, France.
FAU - Agli, B
AU  - Agli B
FAU - Mur, B
AU  - Mur B
FAU - Dieudonne, M N
AU  - Dieudonne MN
FAU - Giudicelli, Y
AU  - Giudicelli Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Androgens)
RN  - 0 (Lipids)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Adipocytes/cytology/*enzymology
MH  - Adipose Tissue/anatomy & histology/*enzymology
MH  - Androgens/*physiology
MH  - Animals
MH  - Blotting, Western
MH  - Body Weight/physiology
MH  - Connective Tissue/enzymology
MH  - Epididymis/enzymology
MH  - Lipids/biosynthesis
MH  - Male
MH  - Orchiectomy
MH  - Protein Kinase C/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1677/joe.0.1380493 [doi]
PST - ppublish
SO  - J Endocrinol. 1993 Sep;138(3):493-501. doi: 10.1677/joe.0.1380493.