PMID- 8279946
OWN - NLM
STAT- MEDLINE
DCOM- 19940208
LR  - 20220316
IS  - 0004-0010 (Print)
IS  - 0004-0010 (Linking)
VI  - 129
IP  - 1
DP  - 1994 Jan
TI  - Exudative neutrophils. Modulation of microbicidal function in the inflammatory 
      microenvironment.
PG  - 99-105
AB  - OBJECTIVE: To determine whether the inflammatory microenvironment primes 
      neutrophils for increased microbicidal activity. DESIGN: In vitro studies of host 
      defense were performed on surgical patients. SETTING: A tertiary care, university 
      hospital. PATIENTS: A volunteer sample of hospitalized preoperative, noninfected 
      surgical patients. INTERVENTION: Exudative neutrophils were collected from 
      skin-blister chambers and functionally compared with circulating neutrophils. 
      METHODS: Flow cytometry was used to evaluate directly neutrophil microbicidal 
      activity (using fluoresceinated Candida albicans), 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide production (using 
      123 dihydrorhodamine), and surface expression of CD11b, CD16, and the fMLP 
      receptor. In vitro tumor necrosis factor alpha was used to determine the 
      possibility and extent of further priming in both circulating and exudative 
      neutrophils. RESULTS: Exudative polymorphonuclear neutrophils have enhanced 
      microbicidal activity, superoxide production, and expression of CD11b, CD16, and 
      the fMLP receptor. Exogenous tumor necrosis factor was able to prime circulating 
      neutrophils but did not further augment superoxide production in exudative 
      neutrophils. CONCLUSION: The microbicidal activity of neutrophils is enhanced 
      after exudation and is associated with neutrophil priming. The inability of 
      exogenous tumor necrosis factor to further augment superoxide production after 
      exudation suggests that this priming has been maximized.
FAU - Yee, J
AU  - Yee J
AD  - Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, 
      Quebec.
FAU - Giannias, B
AU  - Giannias B
FAU - Kapadia, B
AU  - Kapadia B
FAU - Chartrand, L
AU  - Chartrand L
FAU - Christou, N V
AU  - Christou NV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Surg
JT  - Archives of surgery (Chicago, Ill. : 1960)
JID - 9716528
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Candida albicans/*immunology
MH  - Exudates and Transudates
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/immunology/pharmacology
MH  - Neutrophils/immunology/metabolism/*physiology
MH  - Oxygen/metabolism
MH  - Phagocytosis/drug effects/*immunology
MH  - Receptors, Immunologic/analysis
MH  - Skin Window Technique
MH  - Superoxides/metabolism
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1001/archsurg.1994.01420250111014 [doi]
PST - ppublish
SO  - Arch Surg. 1994 Jan;129(1):99-105. doi: 10.1001/archsurg.1994.01420250111014.