PMID- 8280083
OWN - NLM
STAT- MEDLINE
DCOM- 19940207
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 296 ( Pt 3)
IP  - Pt 3
DP  - 1993 Dec 15
TI  - Molecular cloning and tissue distribution of pig transforming growth factor 
      alpha.
PG  - 837-42
AB  - Transforming growth factor alpha (TGF alpha) was originally identified as a 
      product of tumour tissues and transformed cells in culture. Although it is now 
      clear that expression of this factor is not restricted to neoplastic cells, there 
      remains relatively little information about the sites of expression of TGF alpha 
      in normal tissues. Therefore, an amplified DNA fragment encoding the pig TGF 
      alpha precursor was cloned by reverse transcription-PCR (RT-PCR) using RNA 
      isolated from normal skin tissue as the template. Nucleotide sequence analysis 
      predicts a 160-residue transmembrane polypeptide that differs from the rat, mouse 
      and human TGF alpha precursors at 14, 15 and six sites respectively. The 
      distribution of TGF alpha mRNA in a wide variety of pig tissues was analysed by 
      RT-PCR, using oligonucleotide primers based on the pig TGF alpha cDNA sequence. 
      TGF alpha transcripts were detected in RNA isolated from 17 of the 22 tissues 
      analysed, including four previously unreported sites. Using an antibody raised 
      against a synthetic TGF alpha peptide, we have immunolocalized TGF alpha protein 
      to cells within the red pulp of the spleen and to the distal convoluted tubules 
      of the kidney.
FAU - Vaughan, T J
AU  - Vaughan TJ
AD  - Department of Cellular Physiology, A.F.R.C. Institute of Animal Physiology and 
      Genetics Research, Babraham, Cambridge, U.K.
FAU - James, P S
AU  - James PS
FAU - Pascall, J C
AU  - Pascall JC
FAU - Brown, K D
AU  - Brown KD
LA  - eng
SI  - GENBANK/D17642
SI  - GENBANK/D17799
SI  - GENBANK/D17800
SI  - GENBANK/D17801
SI  - GENBANK/D17802
SI  - GENBANK/X71014
SI  - GENBANK/X71481
SI  - GENBANK/X71774
SI  - GENBANK/X74555
SI  - GENBANK/X76909
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Spleen/*metabolism
MH  - Swine
MH  - Tissue Distribution
MH  - Transforming Growth Factor alpha/*genetics/metabolism
PMC - PMC1137770
EDAT- 1993/12/15 00:00
MHDA- 1993/12/15 00:01
PMCR- 1994/06/15
CRDT- 1993/12/15 00:00
PHST- 1993/12/15 00:00 [pubmed]
PHST- 1993/12/15 00:01 [medline]
PHST- 1993/12/15 00:00 [entrez]
PHST- 1994/06/15 00:00 [pmc-release]
AID - 10.1042/bj2960837 [doi]
PST - ppublish
SO  - Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):837-42. doi: 10.1042/bj2960837.