PMID- 8280791
OWN - NLM
STAT- MEDLINE
DCOM- 19940215
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1225
IP  - 2
DP  - 1994 Jan 11
TI  - Isoenzyme-specific quantitative immunoassays for cytosolic glutathione 
      transferases and measurement of the enzymes in blood plasma from cancer patients 
      and in tumor cell lines.
PG  - 223-30
AB  - Enzyme-linked immunoassays (ELISAs) based on the double-antibody sandwich 
      technique have been developed for the quantitative analysis of the major human 
      cytosolic class Pi, Mu and Alpha glutathione transferases (GSTs). The procedures 
      were optimized with respect to antibody concentration for coating of plates as 
      well as other parameters in order to achieve high sensitivity and accuracy. No 
      cross-reactivity was detected between members of the three different classes of 
      GSTs or among the Mu class GSTs M2-2, M3-3 and M4-4 with the ELISA for GST M1-1. 
      The ELISAs have been applied to establish the cytosolic GST profiles of 10 cell 
      lines and to monitor the plasma GST levels in cancer patients. The results 
      revealed that the class Pi GST was the dominant isoenzyme in six (LS 174T, HCT-8, 
      Hu 549 Pat, K-562, U-937 and Hu 549) out of nine tumor cell lines and 
      immortalized hepatocytes (Chang Liver). The isoenzymes A1-1 and M1-1 were 
      determined to be the major GST components in Hep G2 and HeLa cells, respectively. 
      In a clinical study, the majority of the patients with urinary bladder cancer 
      were found to have increased plasma levels of both GST A1-1 and GST P1-1 (10/15), 
      while patients with renal cancer frequently showed increases only in GST P1-1 
      (5/8). The results demonstrate that the ELISAs are suitable for analyzing GST 
      phenotypes in both normal and tumor cells and in monitoring plasma levels of GSTs 
      in cancer patients.
FAU - Hao, X Y
AU  - Hao XY
AD  - Department of Biochemistry, Uppsala University, Sweden.
FAU - Castro, V M
AU  - Castro VM
FAU - Bergh, J
AU  - Bergh J
FAU - Sundstrom, B
AU  - Sundstrom B
FAU - Mannervik, B
AU  - Mannervik B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Isoenzymes)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Cross Reactions
MH  - Cytosol/enzymology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - Glutathione Transferase/*analysis/blood
MH  - Humans
MH  - Isoenzymes/*analysis/blood
MH  - Male
MH  - Neoplasms/*blood
MH  - Phenotype
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured/enzymology/ultrastructure
EDAT- 1994/01/11 00:00
MHDA- 1994/01/11 00:01
CRDT- 1994/01/11 00:00
PHST- 1994/01/11 00:00 [pubmed]
PHST- 1994/01/11 00:01 [medline]
PHST- 1994/01/11 00:00 [entrez]
AID - 0925-4439(94)90082-5 [pii]
AID - 10.1016/0925-4439(94)90082-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Jan 11;1225(2):223-30. doi: 
      10.1016/0925-4439(94)90082-5.