PMID- 8281186
OWN - NLM
STAT- MEDLINE
DCOM- 19940217
LR  - 20190904
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 4
IP  - 6
DP  - 1993 Dec
TI  - Identification of photo-inactive phytochrome A in etiolated seedlings and 
      photo-active phytochrome B in green leaves of the aurea mutant of tomato.
PG  - 1035-42
AB  - The contents of spectrophotometrically measurable phytochrome A (PhyA) and 
      phytochrome B (PhyB) and the corresponding immunochemically detectable 
      apoproteins (PHYA and PHYB) were examined in dark- and light-grown tissues of the 
      aurea mutant of tomato and its wild-type (WT). The amount of PHYA in etiolated 
      aurea seedlings was found to be about 20% of that in the WT; this PHYA showed no 
      photo-reversible changes in absorbance, no downregulation of the level of PHYA in 
      light-grown seedlings, and no differential proteolysis of Pr and Pfr species in 
      vitro which was seen in the case of the WT. By contrast, the amount of PHYB in 
      aurea seedlings was not significantly different from that in WT seedlings. 
      Phytochrome isolated from green leaves of the aurea mutant and purified by 
      ion-exchange chromatography showed a red/far-red reversible spectral change, and 
      its elution profile during chromatography was essentially similar to that of 
      PHYB. The results indicate that aurea is a mutant that is deficient in 
      photoactive PhyA at the etiolated stage, when it contains a spectrally inactive 
      PHYA. However, the mutant contains spectrally active PhyB in its green tissue as 
      does the WT.
FAU - Sharma, R
AU  - Sharma R
AD  - Laboratory of Plant Biological Regulation, Frontier Research Program, RIKEN, 
      Saitama, Japan.
FAU - Lopez-Juez, E
AU  - Lopez-Juez E
FAU - Nagatani, A
AU  - Nagatani A
FAU - Furuya, M
AU  - Furuya M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Phytochrome A)
RN  - 0 (Transcription Factors)
RN  - 11121-56-5 (Phytochrome)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
GS  - phyA
GS  - phyB
MH  - Enzyme Activation
MH  - Immunoblotting
MH  - Light
MH  - Mutation
MH  - *Photoreceptor Cells
MH  - Phytochrome/*analysis/antagonists & inhibitors
MH  - Phytochrome A
MH  - Phytochrome B
MH  - Spectrum Analysis
MH  - *Transcription Factors
MH  - Vegetables/embryology/*enzymology/genetics
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1046/j.1365-313x.1993.04061035.x [doi]
PST - ppublish
SO  - Plant J. 1993 Dec;4(6):1035-42. doi: 10.1046/j.1365-313x.1993.04061035.x.