PMID- 8281451
OWN - NLM
STAT- MEDLINE
DCOM- 19940214
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 626
IP  - 1-2
DP  - 1993 Oct 29
TI  - Brain-derived neurotrophic factor enhances function rather than survival of 
      intrastriatal dopamine cell-rich grafts.
PG  - 37-44
AB  - Brain-derived neurotrophic factor (BDNF) has been shown to promote the survival 
      of dopaminergic neurons from the substantia nigra in cell culture. In order to 
      assess whether a similar survival-promoting effect is present also in vivo, we 
      grafted fetal nigral tissue to the dopamine-depleted striatum of 
      6-hydroxydopamine-lesioned rats receiving two-week intraventricular infusions or 
      daily intrastriatal injections of BDNF, NGF, or vehicle. When infused chronically 
      at a high dose (12 micrograms/day) into the lateral ventricle, BDNF caused a 
      behavioral syndrome of reduced food and water intake, body weight loss, and 
      locomotor hyperactivity in comparison to NGF- and vehicle-infused graft 
      recipients. NGF-infused graft recipients displayed a transient weight loss during 
      the first week of infusion. At 15 days, amphetamine-induced turning was 
      significantly attenuated to 3% of pregraft values in BDNF-infused recipients, 
      whereas functional graft effects were not present in NGF- or vehicle-infused 
      animals. Survival of tyrosine hydroxylase-immunoreactive graft cells, however, 
      was similar in all treatment groups. Notably, NGF- and BDNF-infusions led to a 
      significant size increase of cholinergic host neurons in the medial septal 
      nucleus and the vertical limb of the diagonal band ipsilateral to the infusion, 
      whereas there was no cholinergic neuron hypertrophy in vehicle-infused animals. 
      Daily intrastriatal injections of BDNF (2 micrograms) produced no weight loss or 
      locomotor hyperactivity, but also enhanced functional graft effects in 
      BDNF-injected, as compared to vehicle-injected animals. Survival rates of grafted 
      tyrosine hydroxylase-immunoreactive cells were, however, similar in both 
      groups.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Sauer, H
AU  - Sauer H
AD  - Department of Medical Cell Research, University of Lund, Sweden.
FAU - Fischer, W
AU  - Fischer W
FAU - Nikkhah, G
AU  - Nikkhah G
FAU - Wiegand, S J
AU  - Wiegand SJ
FAU - Brundin, P
AU  - Brundin P
FAU - Lindsay, R M
AU  - Lindsay RM
FAU - Bjorklund, A
AU  - Bjorklund A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - CK833KGX7E (Amphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Amphetamine
MH  - Animals
MH  - Behavior, Animal/*drug effects
MH  - Body Weight/drug effects
MH  - Brain Tissue Transplantation
MH  - Brain-Derived Neurotrophic Factor
MH  - Cerebral Ventricles
MH  - Corpus Striatum/cytology/*drug effects
MH  - Dopamine/*physiology
MH  - Female
MH  - Fetal Tissue Transplantation
MH  - Graft Survival/*drug effects
MH  - Infusions, Parenteral
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurons/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1993/10/29 00:00
MHDA- 1993/10/29 00:01
CRDT- 1993/10/29 00:00
PHST- 1993/10/29 00:00 [pubmed]
PHST- 1993/10/29 00:01 [medline]
PHST- 1993/10/29 00:00 [entrez]
AID - 0006-8993(93)90560-A [pii]
AID - 10.1016/0006-8993(93)90560-a [doi]
PST - ppublish
SO  - Brain Res. 1993 Oct 29;626(1-2):37-44. doi: 10.1016/0006-8993(93)90560-a.