PMID- 8282818
OWN - NLM
STAT- MEDLINE
DCOM- 19940217
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 93
IP  - 1
DP  - 1994 Jan
TI  - Lupus autoantibodies to native DNA cross-react with the A and D SnRNP 
      polypeptides.
PG  - 443-9
AB  - Antibodies to native DNA (nDNA) in sera from patients with systemic lupus 
      erythematosus have been found to frequently correlate with antibodies to the A 
      and D SnRNP proteins measured in Western blot assays. 40 of 54 SLE (74.1%) sera 
      with anti-nDNA bound to A and D proteins, while 9 of 113 sera (8%) without 
      anti-nDNA bound the A and D proteins, P < 10(-8) by Fisher's exact test. 
      Antibodies to nDNA correlated closely with anti-A and anti-D in seven of eight 
      patients followed sequentially, r = 0.7865. Nine human polyclonal anti-nDNA 
      populations were isolated from DNA cellulose columns. Seven reacted equally with 
      A and D, and two reacted predominantly with D. Two of three murine monoclonal 
      anti-DNA antibodies isolated from NZB/NZW F1 hybrid mice bound A and D equally in 
      Western blot with a titer > 1/40,000. These reactions were directed to the 
      unfolded A and D proteins measurable in Western blot since these monoclonals (and 
      several of the human anti-nDNA populations) failed to react with native U1RNP in 
      ELISA or in RNA immunoprecipitation experiments. These newly recognized cross 
      reactions of anti-nDNA may amplify the immune response to DNA and be part of the 
      original immunogenic drive.
FAU - Reichlin, M
AU  - Reichlin M
AD  - Arthritis/Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 
      73104.
FAU - Martin, A
AU  - Martin A
FAU - Taylor-Albert, E
AU  - Taylor-Albert E
FAU - Tsuzaka, K
AU  - Tsuzaka K
FAU - Zhang, W
AU  - Zhang W
FAU - Reichlin, M W
AU  - Reichlin MW
FAU - Koren, E
AU  - Koren E
FAU - Ebling, F M
AU  - Ebling FM
FAU - Tsao, B
AU  - Tsao B
FAU - Hahn, B H
AU  - Hahn BH
LA  - eng
GR  - P01 AI21568/AI/NIAID NIH HHS/United States
GR  - R01 AR 32214/AR/NIAMS NIH HHS/United States
GR  - R01 AR31133/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Autoantibodies/*blood/isolation & purification
MH  - Blotting, Western
MH  - Chromatography, Affinity
MH  - Cross Reactions
MH  - DNA/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Systemic/blood/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred Strains/immunology
MH  - Molecular Weight
MH  - Ribonucleoproteins, Small Nuclear/*immunology
PMC - PMC293809
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
PMCR- 1994/01/01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PHST- 1994/01/01 00:00 [pmc-release]
AID - 10.1172/JCI116980 [doi]
PST - ppublish
SO  - J Clin Invest. 1994 Jan;93(1):443-9. doi: 10.1172/JCI116980.