PMID- 8283137
OWN - NLM
STAT- MEDLINE
DCOM- 19940216
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 55
IP  - 1
DP  - 1994 Jan
TI  - Ligation of N-acetylgalactosamine-containing structures on rat bone marrow cells 
      enhances myeloid differentiation and murine granulocyte-macrophage 
      colony-stimulating factor-induced proliferation.
PG  - 127-32
AB  - Previously we have reported the differentiation-dependent expression of a soybean 
      agglutinin (SBA)-binding structure on rat bone marrow cells (BMCs) during their 
      differentiation into macrophages (m phi s). In the present study we tried to 
      analyze the functional role of the SBA-binding structure in BMC proliferation and 
      differentiation. Addition of SBA to BMC cultures driven into m phi 
      differentiation by recombinant murine granulocyte-macrophage colony-stimulating 
      factor (rmGM-CSF), resulted in a two- to threefold increased proliferation rate 
      compared with rmGM-CSF alone. However, the number of colonies in methyl cellulose 
      was not increased by SBA. The effect of SBA was dose dependent (from 4 to 83 pM 
      SBA), with a maximum effect at 83 pM. Experiments to detect a possible 
      synergistic effect of additional cytokines produced by BMC after SBA treatment 
      were inconclusive. The enhancing effect of SBA was also seen when high-density 
      cells, which did not proliferate in response to rmGM-CSF (mainly granulocytes), 
      were removed. Therefore, SBA may increase the CSF reactivity of responsive m phi 
      progenitor cells directly by binding to N-acetylgalactosamine residues on their 
      surface.
FAU - Krugluger, W
AU  - Krugluger W
AD  - Institute of General and Experimental Pathology, University of Vienna, Austria.
FAU - Koller, M
AU  - Koller M
FAU - Allmaier, M
AU  - Allmaier M
FAU - Boltz-Nitulescu, G
AU  - Boltz-Nitulescu G
FAU - Forster, O
AU  - Forster O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-6)
RN  - 0 (Lectins)
RN  - 0 (Plant Lectins)
RN  - 0 (Soybean Proteins)
RN  - 0 (soybean lectin)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9007-49-2 (DNA)
RN  - KM15WK8O5T (Acetylgalactosamine)
SB  - IM
MH  - Acetylgalactosamine/*physiology
MH  - Animals
MH  - *Bone Marrow Cells
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Division/drug effects
MH  - DNA/biosynthesis
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*physiology
MH  - Hematopoiesis
MH  - Interleukin-3/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Lectins/metabolism/pharmacology
MH  - Male
MH  - *Plant Lectins
MH  - Rats
MH  - Rats, Inbred Lew
MH  - *Soybean Proteins
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/jlb.55.1.127 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1994 Jan;55(1):127-32. doi: 10.1002/jlb.55.1.127.