PMID- 8283973
OWN - NLM
STAT- MEDLINE
DCOM- 19940214
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 125
IP  - 2
DP  - 1993 Aug 25
TI  - Myocardial expression of atrial natriuretic factor gene in early stages of 
      hamster cardiomyopathy.
PG  - 179-92
AB  - Ventricular cardiomyocytes represent the most important source of atrial 
      natriuretic factor (ANF) in pathological conditions such as congestive heart 
      failure (CHF). It has been suggested that in cardiomyopathic Syrian hamster 
      ventricles the ANF gene can be reactivated during the hypertrophic stage 
      occurring before heart failure. The present study was undertaken to investigate 
      ANF gene expression during early stages of myocardial damage and its distribution 
      throughout atrial and ventricular myocardium in UM-X7.1 cardiomyopathic Syrian 
      hamsters (CMPH) before hypertrophy and cardiac failure occur. Atria, right and 
      left ventricles, and interventricular septum of hearts of 20-23 days old (young) 
      and 90-95 days old (adult) CMPH were studied. The absence of hypertrophy and 
      cardiac failure was preliminarly ascertained by microscopic and hemodynamic 
      evaluation. ANF-mRNA as well as tissue and plasma immunoreactive ANF were 
      assayed. Moreover, ANF secretion pattern was evaluated by immunocytochemical 
      techniques. Young and adult CMPH hearts were in the necrotic stage of myocardial 
      disease, as demonstrated by histopathological evaluation and by decreased wet 
      weights (mg/g body weight) of different heart regions. Hemodynamic assessment 
      showed no significant changes of left ventricular end-diastolic pressure (LVEDP) 
      and a decrease of the left ventricular peak systolic pressure (LVSP) and +dP/dt. 
      Plasma immunoreactive ANF (IR-ANF) levels were higher in young (3-fold) and adult 
      (6-fold) CMPH than in age-matched normal hamsters. A reduced IR-ANF concentration 
      (per milligram protein) was observed in both young and adult cardiomyopathic 
      atria in respect to healthy controls, whereas a higher IR-ANF concentration was 
      present in ventricles. A 3-fold, 6-fold and 20-fold increase of IR-ANF 
      concentration was found in right ventricular free-wall (RV), left ventricular 
      free-wall (LV) and interventricular septum (IVS), respectively. Northern-blot 
      analysis confirmed that IVS was the major site of ventricular ANF-mRNA 
      transcription in both young and adult CMPH. ANF-mRNA was increased also in atria 
      where a faster peptide secretion can be hypothesized to lower tissue IR-ANF 
      concentration. ANF secretion in ventricular myocardium was achieved via 
      constitutive pathway as demonstrated by immunocytochemistry. Different patterns 
      of ANF gene reactivation occur in CMPH myocardium before intraventricular 
      pressure increases and structural hypertrophic modifications are detectable. The 
      extent of ANF gene reactivation in CMPH ventricles parallels the severity of 
      necrotic damage. Moreover, ANF gene expression is heterogeneously distributed 
      throughout the myocardium, suggesting that interventricular septum, the 
      ontogenically youngest heart region, might preserve foetal characters which can 
      be rapidly reactivated in pathological conditions.
FAU - Di Nardo, P
AU  - Di Nardo P
AD  - Department of Internal Medicine, University of Rome Tor Vergata, Italy.
FAU - Minieri, M
AU  - Minieri M
FAU - Carbone, A
AU  - Carbone A
FAU - Maggiano, N
AU  - Maggiano N
FAU - Micheletti, R
AU  - Micheletti R
FAU - Peruzzi, G
AU  - Peruzzi G
FAU - Tallarida, G
AU  - Tallarida G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (RNA, Messenger)
RN  - 85637-73-6 (Atrial Natriuretic Factor)
SB  - IM
MH  - Animals
MH  - Atrial Natriuretic Factor/analysis/*genetics
MH  - Cardiomyopathies/*metabolism/pathology
MH  - Cricetinae
MH  - Gene Expression
MH  - Heart Ventricles/chemistry
MH  - Hemodynamics/physiology
MH  - Immunohistochemistry
MH  - Mesocricetus
MH  - Myocardium/*metabolism
MH  - RNA, Messenger/analysis
MH  - Random Allocation
MH  - Reference Values
MH  - Time Factors
EDAT- 1993/08/25 00:00
MHDA- 1993/08/25 00:01
CRDT- 1993/08/25 00:00
PHST- 1993/08/25 00:00 [pubmed]
PHST- 1993/08/25 00:01 [medline]
PHST- 1993/08/25 00:00 [entrez]
AID - 10.1007/BF00936447 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1993 Aug 25;125(2):179-92. doi: 10.1007/BF00936447.