PMID- 8284286
OWN - NLM
STAT- MEDLINE
DCOM- 19940217
LR  - 20190904
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 13
IP  - 8
DP  - 1993 Aug
TI  - Molecular cytogenetic analysis of term placentae suspected of mosaicism using 
      fluorescence in situ hybridization.
PG  - 671-9
AB  - In first-trimester chorionic villus sampling (CVS) for prenatal diagnosis, 
      abnormal chromosomal findings, such as mosaicism, trisomies, or suspect abnormal 
      karyotypes, are found more frequently than at amniocentesis. The fact that these 
      chromosomal abnormalities do not always reflect the fetal karyotype but may be 
      restricted to the placenta is a major problem in diagnosis and counselling. In 
      this paper we present the results of fluorescence in situ hybridization (FISH) 
      studies on interphase nuclei of three term placentae investigated because of 
      false-positive findings at first-trimester CVS. The chorionic villi of the first 
      case showed a mosaic chromosome pattern involving a trisomy 10 cell line and a 
      normal cell line, those of the second case a total trisomy 8 cell line, while in 
      the third case a complete monosomy X was found. Follow-up amniocentesis in each 
      of these three cases revealed a normal karyotype. By using FISH, we were able to 
      confirm the presence of the aberrant cell lines, which were all confined to one 
      part of the placenta. FISH on interphase nuclei allows the investigation of large 
      numbers of cells for the existence of numerical chromosome aberrations in a quick 
      and reliable way.
FAU - Schuring-Blom, G H
AU  - Schuring-Blom GH
AD  - Institute of Human Genetics, University of Amsterdam, The Netherlands.
FAU - Keijzer, M
AU  - Keijzer M
FAU - Jakobs, M E
AU  - Jakobs ME
FAU - Van den Brande, D M
AU  - Van den Brande DM
FAU - Visser, H M
AU  - Visser HM
FAU - Wiegant, J
AU  - Wiegant J
FAU - Hoovers, J M
AU  - Hoovers JM
FAU - Leschot, N J
AU  - Leschot NJ
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - Chorionic Villi Sampling
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Mosaicism
MH  - Placenta/*physiology
MH  - Pregnancy
MH  - Pregnancy Trimester, Third
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 10.1002/pd.1970130803 [doi]
PST - ppublish
SO  - Prenat Diagn. 1993 Aug;13(8):671-9. doi: 10.1002/pd.1970130803.