PMID- 8286380
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 33
IP  - 2
DP  - 1994 Jan 18
TI  - Picosecond infrared study of the photodynamics of carbonmonoxy-cytochrome c 
      oxidase.
PG  - 500-7
AB  - Time-resolved infrared (TRIR) techniques have been employed to study the 
      reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome 
      c oxidase (CcO). The ligation dynamics immediately following photodissociation 
      have been investigated using picosecond TRIR spectroscopy and linear dichroism. 
      The rate of photoinitiated transfer of CO from cytochrome alpha 3 to CuB was 
      measured directly by monitoring the development of the transient CuBCO 
      absorption. In less than 1 ps, a stationary CuBCO spectrum develops, which 
      together with the CO infrared linear dichroism is constant until the CO 
      dissociates from CuB on a microsecond time scale. These observations indicate 
      that the CO is transferred between metals and reaches its equilibrium 
      conformation in less than 1 ps. This unprecedented ligand transfer rate has 
      profound implications with regard to the structure and dynamics of the cytochrome 
      alpha 3-CuB site, the functional architecture of the protein, and coordination 
      dynamics in general.
FAU - Dyer, R B
AU  - Dyer RB
AD  - Chemical Sciences and Technology Division (CST), Los Alamos National Laboratory, 
      New Mexico 87545.
FAU - Peterson, K A
AU  - Peterson KA
FAU - Stoutland, P O
AU  - Stoutland PO
FAU - Woodruff, W H
AU  - Woodruff WH
LA  - eng
GR  - GM 45807/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 7U1EE4V452 (Carbon Monoxide)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Carbon Monoxide/chemistry/*metabolism
MH  - Cattle
MH  - Electron Transport Complex IV/*chemistry/*metabolism
MH  - Molecular Conformation
MH  - Molecular Structure
MH  - Photochemistry
MH  - *Spectrophotometry, Infrared
MH  - Time Factors
EDAT- 1994/01/18 00:00
MHDA- 1994/01/18 00:01
CRDT- 1994/01/18 00:00
PHST- 1994/01/18 00:00 [pubmed]
PHST- 1994/01/18 00:01 [medline]
PHST- 1994/01/18 00:00 [entrez]
AID - 10.1021/bi00168a015 [doi]
PST - ppublish
SO  - Biochemistry. 1994 Jan 18;33(2):500-7. doi: 10.1021/bi00168a015.