PMID- 8286584
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 49
IP  - 5
DP  - 1993 Nov
TI  - Localization of transforming growth factor alpha in the human placenta and 
      decidua: role in trophoblast growth.
PG  - 885-94
AB  - Transforming growth factor alpha (TGF alpha) is an important growth regulatory 
      molecule, the location and function of which at the human fetomaternal interface 
      remain to be determined. The present study examined the presence of TGF alpha in 
      the human placenta, decidua, and fetal membranes throughout gestation (from a 
      total of 29 subjects) as well as its functional role in the proliferation of 
      first trimester trophoblasts. The peptide was localized immunocytochemically with 
      a monoclonal anti-TGF alpha antibody (Ab) (MF9) on paraffin-embedded tissues via 
      the avidin-biotin complex-peroxidase technique with diaminobenzidine (DAB) as the 
      chromogen. Omission or TGF alpha absorption of the primary Ab served as negative 
      controls. Specific (cytoplasmic) staining was noted in typical stromal-type 
      decidual cells, including cells of the decidua basalis and parietalis and 
      chorionic decidua, throughout gestation. Villous trophoblast cells 
      (syncytiotrophoblast and to a minor extent cytotrophoblast) at all gestational 
      ages as well as extravillous cytotrophoblast cells (intermediate and 
      cytotrophoblastic shell) also showed specific cytoplasmic staining. Chorionic 
      trophoblasts showed variable staining, and little or no immunoreactivity was seen 
      in the amniocytes. Second-passage first trimester human trophoblast cells 
      (characterized by their expression of cytokeratin as well as other markers) were 
      cultured in the presence of TGF alpha or neutralizing anti-TGF alpha Ab (TAb-1) 
      or no additive for 18 h prior to exposure to 3H-TdR for 6 h to measure 3H-TdR 
      uptake. TGF alpha (0-100 ng/ml) caused a dose-dependent stimulation of 
      proliferation, reaching a near plateau at 6-100 ng/ml to slightly more than 
      double the basal level. The presence of anti-TGF alpha Ab alone (25 
      micrograms/ml) did not significantly influence the proliferation of the cells, 
      indicating the absence of significant endogenous TGF alpha in these cultures; 
      however, the Ab was able to abolish the stimulatory function of exogenous TGF 
      alpha. Exogenous TGF alpha also increased the number of trophoblast nuclei 
      immunoreactive for proliferating cell nuclear antigen and reduced the incidence 
      of multinucleate cells in culture. These results indicate that TGF alpha is 
      present in the cells of the fetomaternal interface throughout human gestation and 
      may function as a stimulator of trophoblastic growth in situ.
FAU - Lysiak, J J
AU  - Lysiak JJ
AD  - Department of Anatomy, University of Western Ontario, London, Canada.
FAU - Han, V K
AU  - Han VK
FAU - Lala, P K
AU  - Lala PK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Transforming Growth Factor alpha)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Decidua/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Thymidine/metabolism
MH  - Transforming Growth Factor alpha/*metabolism/pharmacology/physiology
MH  - Trophoblasts/cytology/drug effects/*physiology
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 10.1095/biolreprod49.5.885 [doi]
PST - ppublish
SO  - Biol Reprod. 1993 Nov;49(5):885-94. doi: 10.1095/biolreprod49.5.885.