PMID- 8286609
OWN - NLM
STAT- MEDLINE
DCOM- 19940224
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 49
IP  - 6
DP  - 1993 Dec
TI  - Dynamic changes of gap junctions and cytoskeleton during in vitro culture of 
      cattle oocyte cumulus complexes.
PG  - 1277-87
AB  - Changes in cell-to-cell contact and distribution of cytoskeletal components were 
      investigated during in vitro culture of cattle oocyte cumulus complexes (OCC). 
      Freeze-fracture analysis (FF), microinjections of the fluorescent dye Lucifer 
      Yellow (LY), immunofluorescence, and ultrastructural immunocytochemistry were 
      used. The cumulus cells (CC) remained in close contact via gap junctions (GJ) 
      constituted of connexin43 (Cx43) during the entire culture time. Whereas the GJ 
      decreased in diameter after 24 h of culture, their number was still substantially 
      great at that time. The Cx43-positive GJ, localized between corona radiata cell 
      projections and oolemma, disappeared after 6 h of culture. Concomitantly, the OCC 
      lost the ability to transfer LY from cumulus to oocyte, and connexin32 (Cx32) 
      became detectable in the oocytes. Both the changes in corona-oocyte coupling and 
      cumulus expansion were preceded by the redistribution of F-actin in cytoplasm of 
      CC. These data indicate that functional GJ linked the CC until the second meiotic 
      arrest. However, the removal of Cx43-positive GJ interconnecting cytoplasmic 
      projections of corona radiata cells with the oocyte was temporally correlated 
      with germinal vesicle breakdown. The present results suggest the pivotal role of 
      the cytoskeleton (F-actin) in cumulus expansion.
FAU - Sutovsky, P
AU  - Sutovsky P
AD  - Institute of Physiology and Genetics of Animals, Libechov, Czech Republic.
FAU - Flechon, J E
AU  - Flechon JE
FAU - Flechon, B
AU  - Flechon B
FAU - Motlik, J
AU  - Motlik J
FAU - Peynot, N
AU  - Peynot N
FAU - Chesne, P
AU  - Chesne P
FAU - Heyman, Y
AU  - Heyman Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Actins)
RN  - 0 (Connexin 43)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Isoquinolines)
RN  - 0 (Tubulin)
RN  - 0 (Vimentin)
RN  - 9654F8OVKE (lucifer yellow)
SB  - IM
MH  - Actin Cytoskeleton/ultrastructure
MH  - Actins/analysis
MH  - Animals
MH  - Cattle
MH  - Connexin 43/physiology
MH  - Cytoskeleton/*ultrastructure
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Fluorescent Dyes
MH  - Freeze Fracturing
MH  - Immunohistochemistry
MH  - Intercellular Junctions/*ultrastructure
MH  - Intermediate Filaments/ultrastructure
MH  - Isoquinolines
MH  - Microscopy, Electron
MH  - Microtubules/ultrastructure
MH  - Oocytes/*ultrastructure
MH  - Time Factors
MH  - Tubulin/analysis
MH  - Vimentin/analysis
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1095/biolreprod49.6.1277 [doi]
PST - ppublish
SO  - Biol Reprod. 1993 Dec;49(6):1277-87. doi: 10.1095/biolreprod49.6.1277.