PMID- 8287226
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20041117
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 2
IP  - 1
DP  - 1993 Mar
TI  - Analysis of chromosome aneuploidy in ovarian dysgerminoma by flow cytometry and 
      fluorescence in situ hybridization.
PG  - 50-6
AB  - We determined the DNA ploidy and the centromeric copy number of chromosomes 7, 
      12, 18, and X in four cases of ovarian dysgerminoma using DNA flow cytometry and 
      fluorescence in situ hybridization (FISH) with chromosome-specific 
      alpha-satellite probes. The analyses were performed on nuclei isolated from 
      paraffin-embedded tissue. The DNA index of the tumors ranged from 1.75 to 2.08 
      (near tetraploid). The FISH analysis demonstrated five copies of chromosome 7 and 
      four copies of chromosome 12 in most tumors. The copy number of chromosome 18 
      ranged from two to four. The X chromosome was present in three copies in most 
      tumors. These data show that the aneuploidy profile of dysgerminoma is similar to 
      that of testicular seminoma. Overrepresentation of chromosomes 7 and 12 and 
      under-representation of chromosome 18 are characteristic cytogenetic features of 
      seminoma. Seminoma and dysgerminoma share the same chromosomal marker, an 
      isochromosome i(12p). Our data suggest that these tumors are also characterized 
      by a similar, nonrandom pattern of chromosome gains and losses.
FAU - Gibas, Z
AU  - Gibas Z
AD  - Department of Pathology and Cell Biology, Thomas Jefferson University, 
      Philadelphia, Pennsylvania 19107.
FAU - Talerman, A
AU  - Talerman A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Child
MH  - DNA, Neoplasm/*genetics
MH  - Dysgerminoma/*genetics/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ovarian Neoplasms/*genetics/pathology
EDAT- 1993/03/01 00:00
MHDA- 1993/03/01 00:01
CRDT- 1993/03/01 00:00
PHST- 1993/03/01 00:00 [pubmed]
PHST- 1993/03/01 00:01 [medline]
PHST- 1993/03/01 00:00 [entrez]
PST - ppublish
SO  - Diagn Mol Pathol. 1993 Mar;2(1):50-6.