PMID- 8288872
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 166
IP  - 2
DP  - 1993 Dec 3
TI  - Measurement of eosinophil cationic protein (ECP) and eosinophil protein 
      X/eosinophil derived neurotoxin (EPX/EDN). Time and temperature dependent 
      spontaneous release in vitro demands standardized sample processing.
PG  - 183-90
AB  - The measurement of eosinophil derived proteins such as eosinophil cationic 
      protein (ECP) and eosinophil protein X/eosinophil derived neurotoxin (EPX/EDN) in 
      biological fluids may be a useful indicator of eosinophil activity in ongoing 
      inflammatory processes. This study was performed on blood samples and illustrates 
      that serum values of ECP in particular, but also of EPX, are mainly a result of 
      spontaneous release during the processing of blood samples. In the presence of 
      divalent cations (Ca2+ and Mg2+), the amount of released ECP and EPX is dependent 
      upon the incubation temperature and the time before centrifugation and recovery 
      of serum. Moreover, the utensils used for blood sampling may influence the serum 
      levels of ECP and EPX. Thus, standardized sample processing is of paramount 
      importance if the results are to have optimal diagnostic or clinical value.
FAU - Reimert, C M
AU  - Reimert CM
AD  - Laboratory of Medical Allergology, National University Hospital, Copenhagen, 
      Denmark.
FAU - Poulsen, L K
AU  - Poulsen LK
FAU - Bindslev-Jensen, C
AU  - Bindslev-Jensen C
FAU - Kharazmi, A
AU  - Kharazmi A
FAU - Bendtzen, K
AU  - Bendtzen K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Blood Proteins)
RN  - 0 (Eosinophil Granule Proteins)
RN  - 0 (Neurotoxins)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - EC 3.1.- (Eosinophil-Derived Neurotoxin)
RN  - EC 3.1.- (Ribonucleases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Blood Proteins/*analysis/metabolism
MH  - Blood Specimen Collection
MH  - Calcium/blood
MH  - Edetic Acid/metabolism
MH  - Eosinophil Granule Proteins
MH  - Eosinophil-Derived Neurotoxin
MH  - Hemostatic Techniques
MH  - Humans
MH  - Neurotoxins/*blood/metabolism
MH  - *Ribonucleases
MH  - Temperature
MH  - Time Factors
EDAT- 1993/12/03 00:00
MHDA- 1993/12/03 00:01
CRDT- 1993/12/03 00:00
PHST- 1993/12/03 00:00 [pubmed]
PHST- 1993/12/03 00:01 [medline]
PHST- 1993/12/03 00:00 [entrez]
AID - 0022-1759(93)90359-F [pii]
AID - 10.1016/0022-1759(93)90359-f [doi]
PST - ppublish
SO  - J Immunol Methods. 1993 Dec 3;166(2):183-90. doi: 10.1016/0022-1759(93)90359-f.