PMID- 8289346
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 68
IP  - 2
DP  - 1994 Feb
TI  - Human herpesvirus 6 infects cervical epithelial cells and transactivates human 
      papillomavirus gene expression.
PG  - 1173-8
AB  - To examine whether human herpesvirus 6 (HHV-6) is capable of infecting human 
      cervical epithelial cells and altering expression of human papillomavirus (HPV) 
      genes, HPV-immortalized or -transformed carcinoma cell lines were infected with 
      HHV-6 variant A. No cytopathic effect was observed in infected cervical cells. 
      However, immunofluorescence indicated that infected cells expressed early-late 
      proteins of HHV-6 by day 3 postinfection. HHV-6 DNA was also detected by Southern 
      blot hybridization after infection and persisted through continued subculture in 
      an episomal state as proven by Gardella gel electrophoresis and fluorescence in 
      situ hybridization. HHV-6 infection enhanced expression of HPV RNAs encoding the 
      viral oncoproteins E6 and E7. Transient transfection assays showed that two HHV-6 
      molecular clones, pZVB-70 and pZVH-14, upregulated transcription 9- to 15-fold 
      from a receptor plasmid containing the HPV type 18 regulatory sequences which 
      control transcription in vivo. Cervical carcinoma cells infected with HHV-6 
      induced more rapid development of tumors in mice than did noninfected cells. 
      These results are the first evidence that human cervical epithelial cells can be 
      infected with HHV-6 and that HHV-6 contains transactivators which stimulate the 
      HPV-transforming genes.
FAU - Chen, M
AU  - Chen M
AD  - Laboratory of Biology, National Cancer Institute, Bethesda, Maryland 20892.
FAU - Popescu, N
AU  - Popescu N
FAU - Woodworth, C
AU  - Woodworth C
FAU - Berneman, Z
AU  - Berneman Z
FAU - Corbellino, M
AU  - Corbellino M
FAU - Lusso, P
AU  - Lusso P
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - DiPaolo, J A
AU  - DiPaolo JA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E6 protein, Human papillomavirus type 18)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (oncogene protein E7, Human papillomavirus type 6)
SB  - IM
MH  - Animals
MH  - Carcinoma/*virology
MH  - DNA, Viral/analysis
MH  - *DNA-Binding Proteins
MH  - Epithelium/virology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Mice
MH  - Neoplasms, Experimental
MH  - Oncogene Proteins, Viral/analysis
MH  - Papillomaviridae/*genetics
MH  - *Transcriptional Activation
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*virology
PMC - PMC236556
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
PMCR- 1994/02/01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PHST- 1994/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.68.2.1173-1178.1994 [doi]
PST - ppublish
SO  - J Virol. 1994 Feb;68(2):1173-8. doi: 10.1128/JVI.68.2.1173-1178.1994.