PMID- 8290558
OWN - NLM
STAT- MEDLINE
DCOM- 19940222
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 2
DP  - 1994 Jan 18
TI  - Detection of nondisjunction and recombination in meiotic and postmeiotic cells 
      from XYSxr [XY,Tp(Y)1Ct] mice using multicolor fluorescence in situ 
      hybridization.
PG  - 524-8
AB  - Current meiotic dogma holds that synapsis is required for recombination and that 
      recombination is required for proper disjunction. The mouse chromosome aberration 
      XYSxr [sex reversal; redesignated XY,Tp(Y)1Ct] appears to challenge this 
      assumption, for although chromosomes X and Y often fail to synapse and recombine, 
      there is no dramatic increase in aneuploid progeny. An explanation of this 
      conundrum might be that X-Y univalent spermatocytes do not survive. The phenotype 
      of sex reversal is generated by the "obligatory" crossover between the X and Y 
      chromosomes, which always occurs proximal to a duplicated copy of the 
      testis-determining gene Sry and transfers one copy from one chromatid of the Y 
      chromosome to one chromatid of the X. Animals that inherit an X chromosome with 
      the Sry gene are chromosomally female but phenotypically male. We have used 
      fluorescence in situ hybridization (FISH) to visualize probes for the X and Y 
      chromosomes and for the Sry sequence and chromosome 8 to track the fate of both 
      recombinant and nonrecombinant chromosomes through metaphases I and II into 
      spermatids and sperm. In the 219 gametes examined by multicolor FISH, the 
      frequency of aneuploid products (XY or "O") was low (3.7%) despite a high 
      frequency (66%) of X-Y separation at metaphase I. In balanced gametes, X and Y 
      recombinant chromosomes slightly exceeded nonrecombinants. Both of these 
      observations support the earlier proposal that asynapsis and nondisjunction in 
      primary spermatocytes lead to their developmental arrest and degeneration.
FAU - Ashley, T
AU  - Ashley T
AD  - Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
FAU - Ried, T
AU  - Ried T
FAU - Ward, D C
AU  - Ward DC
LA  - eng
GR  - GM-40115/GM/NIGMS NIH HHS/United States
GR  - HG-00272/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Probes)
SB  - IM
GS  - Sry
MH  - Aneuploidy
MH  - Animals
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - *Disorders of Sex Development
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis/*genetics
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Phenotype
MH  - Recombination, Genetic
MH  - Sex Determination Analysis
MH  - Spermatids/ultrastructure
MH  - Spermatozoa/ultrastructure
MH  - *X Chromosome
MH  - *Y Chromosome
PMC - PMC42981
EDAT- 1994/01/18 00:00
MHDA- 1994/01/18 00:01
PMCR- 1994/07/18
CRDT- 1994/01/18 00:00
PHST- 1994/01/18 00:00 [pubmed]
PHST- 1994/01/18 00:01 [medline]
PHST- 1994/01/18 00:00 [entrez]
PHST- 1994/07/18 00:00 [pmc-release]
AID - 10.1073/pnas.91.2.524 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):524-8. doi: 10.1073/pnas.91.2.524.