PMID- 8290566
OWN - NLM
STAT- MEDLINE
DCOM- 19940222
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 2
DP  - 1994 Jan 18
TI  - Reconstitution of functional human single-stranded DNA-binding protein from 
      individual subunits expressed by recombinant baculoviruses.
PG  - 579-83
AB  - Human single-stranded DNA-binding protein (HSSB), also known as replication 
      protein A, is composed of a 70-kDa single-stranded DNA-binding subunit (p70) and 
      34-kDa and 11-kDa (p34 and p11, respectively) subunits of unknown functions. We 
      have examined interactions among the HSSB subunits in vivo by coinfecting insect 
      cells with different combinations of recombinant baculoviruses encoding p70, p34, 
      or p11. In vivo, coexpressed p34 and p11 subunits formed stable complexes, 
      whereas neither p34 nor p11 formed stable complexes with p70. In cells coinfected 
      with viruses expressing all three subunits, the stable heterotrimer formed, 
      which, when purified, replaced HSSB isolated from HeLa cells in various assays, 
      including simian virus 40 DNA replication in vitro. These data suggest that, in 
      the assembly of functionally active HSSB, formation of the p34-p11 complex 
      precedes p70 addition to the complex.
FAU - Stigger, E
AU  - Stigger E
AD  - Department of Virology and Molecular Biology, St. Jude Children's Research 
      Hospital, Memphis, TN 38101-0318.
FAU - Dean, F B
AU  - Dean FB
FAU - Hurwitz, J
AU  - Hurwitz J
FAU - Lee, S H
AU  - Lee SH
LA  - eng
GR  - 5P30 CA 21765-15/CA/NCI NIH HHS/United States
GR  - 5P30 CA 21765-16/CA/NCI NIH HHS/United States
GR  - 5R01 GM34559/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Baculoviridae/genetics
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Replication
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/metabolism
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Moths
MH  - Protein Conformation
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Simian virus 40/metabolism
PMC - PMC42992
EDAT- 1994/01/18 00:00
MHDA- 1994/01/18 00:01
PMCR- 1994/07/18
CRDT- 1994/01/18 00:00
PHST- 1994/01/18 00:00 [pubmed]
PHST- 1994/01/18 00:01 [medline]
PHST- 1994/01/18 00:00 [entrez]
PHST- 1994/07/18 00:00 [pmc-release]
AID - 10.1073/pnas.91.2.579 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):579-83. doi: 10.1073/pnas.91.2.579.