PMID- 8291189
OWN - NLM
STAT- MEDLINE
DCOM- 19940222
LR  - 20190825
IS  - 0304-4017 (Print)
IS  - 0304-4017 (Linking)
VI  - 50
IP  - 1-2
DP  - 1993 Oct
TI  - A colorimetric assay for trypanosome viability and metabolic function.
PG  - 143-9
AB  - We have adapted a tetrazolium salt (MTT) colorimetric cytotoxicity assay to the 
      assessment of viability and metabolic function in cultured African trypanosomes. 
      Trypomastigotes of Trypanosoma congolense and T. brucei rhodesianse were 
      harvested from the blood of parasitemic rats and cultured under axenic conditions 
      that support trypanosome viability and growth. Analysis of serial dilutions of 
      these bloodstream forms indicated that the assay could detect 10(4) parasites. To 
      assess the effect of lymphoid cytokines on trypomastigote viability, 10(5) 
      freshly harvested parasites were cultured with a wide range of dilutions of human 
      recombinant IL-1, IL-3, IL-6, interferon-gamma (IFN gamma) or tumor necrosis 
      factor-alpha (TNF alpha), or bovine recombinant IFN gamma or TNF alpha for 24, 48 
      or 96 h. These cytokines had no apparent growth enhancing or inhibitory effect on 
      the trypomastigotes compared with growth in supplemented medium alone. This assay 
      has several advantages over traditional counting methods, including increased 
      sensitivity and rapid, repeatable quantitation. This adaptation of the MTT 
      colorimetric assay should be useful in screening drugs and host-derived factors 
      for growth-modulating effects on trypanosomes and other extracellular protozoan 
      parasites.
FAU - Ellis, J A
AU  - Ellis JA
AD  - International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
FAU - Fish, W R
AU  - Fish WR
FAU - Sileghem, M
AU  - Sileghem M
FAU - McOdimba, F
AU  - McOdimba F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Parasitol
JT  - Veterinary parasitology
JID - 7602745
RN  - 0 (Coloring Agents)
RN  - 0 (Cytokines)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Colorimetry
MH  - Coloring Agents
MH  - Cytokines/pharmacology
MH  - Male
MH  - Oxidation-Reduction
MH  - Rats
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Trypanosoma brucei rhodesiense/drug effects/*growth & development/metabolism
MH  - Trypanosoma congolense/drug effects/*growth & development/metabolism
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0304-4017(93)90015-F [pii]
AID - 10.1016/0304-4017(93)90015-f [doi]
PST - ppublish
SO  - Vet Parasitol. 1993 Oct;50(1-2):143-9. doi: 10.1016/0304-4017(93)90015-f.