PMID- 8292041
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20141120
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 198
IP  - 1
DP  - 1994 Jan 14
TI  - Attenuation of human leukocyte adherence to endothelial cell monolayers by 
      tyrosine kinase inhibitors.
PG  - 359-65
AB  - Expression of cell adhesion molecules is controlled by various cytokines 
      including interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF alpha), but 
      the underlying mechanisms are not clear. In this study, we investigated the role 
      of tyrosine phosphorylated proteins in the regulation of leukocyte adhesion to 
      endothelial cells. We examined the effect of genistein and herbimycin-A, 
      selective inhibitors of tyrosine kinase, on the adherence of human blood 
      neutrophils, lymphocytes, and monocytes to monolayers of human umbilical vein 
      endothelial cells. Both genistein and herbimycin A significantly inhibited IL-1 
      and TNF alpha-induced upregulation of neutrophils (p < 0.05) and monocyte (p < 
      0.01) adherence. IL-1 and TNF alpha-stimulated lymphocyte adherence was 
      diminished in the presence of herbimycin A (p < 0.05), but genistein only 
      inhibited TNF alpha-stimulated adherence. There was no significant effect of 
      genistein on IL-1-induced lymphocyte adherence. These novel findings reveal for 
      the first time that tyrosine phosphorylated proteins may regulate leukocyte 
      adherence and cell adhesion molecule expression on the endothelium.
FAU - McGregor, P E
AU  - McGregor PE
AD  - Department of Surgery, Creighton University School of Medicine, Omaha, NE 68178.
FAU - Agrawal, D K
AU  - Agrawal DK
FAU - Edwards, J D
AU  - Edwards JD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Benzoquinones)
RN  - 0 (Interleukin-1)
RN  - 0 (Isoflavones)
RN  - 0 (Lactams, Macrocyclic)
RN  - 0 (Phosphoproteins)
RN  - 0 (Quinones)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1W306TDA6S (Rifabutin)
RN  - 70563-58-5 (herbimycin)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Analysis of Variance
MH  - Benzoquinones
MH  - Cell Adhesion/*drug effects
MH  - Cells, Cultured
MH  - Endothelium, Vascular/drug effects/*physiology
MH  - Genistein
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Isoflavones/*pharmacology
MH  - Lactams, Macrocyclic
MH  - Leukocytes/drug effects/*physiology
MH  - Lymphocytes/drug effects/physiology
MH  - Monocytes/drug effects/physiology
MH  - Neutrophils/drug effects/physiology
MH  - Phosphoproteins/*metabolism
MH  - Protein-Tyrosine Kinases/*antagonists & inhibitors
MH  - Quinones/*pharmacology
MH  - Rifabutin/analogs & derivatives
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Umbilical Veins
EDAT- 1994/01/14 00:00
MHDA- 1994/01/14 00:01
CRDT- 1994/01/14 00:00
PHST- 1994/01/14 00:00 [pubmed]
PHST- 1994/01/14 00:01 [medline]
PHST- 1994/01/14 00:00 [entrez]
AID - S0006-291X(84)71050-3 [pii]
AID - 10.1006/bbrc.1994.1050 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Jan 14;198(1):359-65. doi: 
      10.1006/bbrc.1994.1050.