PMID- 8292044
OWN - NLM
STAT- MEDLINE
DCOM- 19940218
LR  - 20101118
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 198
IP  - 1
DP  - 1994 Jan 14
TI  - Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and 
      characterization of the enzyme.
PG  - 380-6
AB  - Using genetic mapping approaches, a gene on chromosome 10, Bp1, has been 
      identified in the stroke-prone spontaneously hypertensive rat (SHRSP) in the same 
      region that contains the gene for angiotensin converting enzyme (ACE). Since ACE 
      plays an important role in blood pressure regulation, the ACE gene is a leading 
      candidate for Bp1. To examine the possibility that a structural abnormality of 
      ACE exists in the SHRSP, we cloned and characterized the cDNAs for the 
      Wistar-Kyoto rat (WKY) and SHRSP ACE. Both cDNAs encode a single polypeptide of 
      1,313 amino acid residues with an estimated molecular weight of 150.9 KDa. Five 
      nucleotide differences were identified between the WKY and the SHRSP ACE cDNAs. 
      One of these differences resulted in an amino acid substitution (Lys-207 in the 
      WKY to Arg-207 in the SHRSP). But the enzymatic properties of partially purified 
      ACE from the two strains were similar. Thus the data suggest that an alteration 
      in the primary structure of rat ACE does not contribute to the hypertension in 
      the SHRSP.
FAU - Koike, G
AU  - Koike G
AD  - Falk Cardiovascular Research Center, Stanford University School of Medicine, CA 
      94305-5246.
FAU - Krieger, J E
AU  - Krieger JE
FAU - Jacob, H J
AU  - Jacob HJ
FAU - Mukoyama, M
AU  - Mukoyama M
FAU - Pratt, R E
AU  - Pratt RE
FAU - Dzau, V J
AU  - Dzau VJ
LA  - eng
SI  - GENBANK/U03708
SI  - GENBANK/U03734
GR  - HL 35252/HL/NHLBI NIH HHS/United States
GR  - HL 35610/HL/NHLBI NIH HHS/United States
GR  - HL 46631/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
SB  - IM
GS  - Bp1
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Evolution
MH  - Cerebrovascular Disorders/genetics
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - Conserved Sequence
MH  - DNA Primers
MH  - DNA, Complementary/*metabolism
MH  - Gene Library
MH  - Humans
MH  - Hypertension/enzymology/*genetics
MH  - Kinetics
MH  - Lung/enzymology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptidyl-Dipeptidase A/biosynthesis/*genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Inbred SHR/*genetics
MH  - Rats, Inbred WKY/genetics
MH  - Restriction Mapping
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1994/01/14 00:00
MHDA- 1994/01/14 00:01
CRDT- 1994/01/14 00:00
PHST- 1994/01/14 00:00 [pubmed]
PHST- 1994/01/14 00:01 [medline]
PHST- 1994/01/14 00:00 [entrez]
AID - S0006-291X(84)71053-9 [pii]
AID - 10.1006/bbrc.1994.1053 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Jan 14;198(1):380-6. doi: 
      10.1006/bbrc.1994.1053.