PMID- 8297479
OWN - NLM
STAT- MEDLINE
DCOM- 19940310
LR  - 20191023
IS  - 0899-1987 (Print)
IS  - 0899-1987 (Linking)
VI  - 9
IP  - 1
DP  - 1994 Jan
TI  - Transforming growth factor-beta 1 expression in Syrian hamster cheek pouch 
      carcinogenesis.
PG  - 10-6
AB  - The expression pattern of transforming growth factor-beta 1 (TGF-beta 1) during 
      the stages of complete carcinogenesis in the hamster cheek pouch model was 
      studied. The right cheek pouches of 18 male hamsters were treated with 0.5%, 
      7,12-dimethylbenz[a]anthracene (DMBA) for 16 wk. TGF-beta 1 was detected 
      immunohistochemically in the resulting samples with two different polyclonal 
      monospecific antibodies that recognize intracellular and extracellular forms of 
      TGF-beta 1. In the normal cheek pouch, extracellular protein stained the corium 
      strongly, but the reaction was not evenly distributed. As treatment progressed, 
      the reaction increased in both area and intensity; the peak was reached at 8 wk. 
      Intracellular TGF-beta 1 expression followed a similar pattern, with a peak at 4 
      wk of treatment. The results of northern blot analysis were concordant with the 
      immunohistochemical results. Overexpression of TGF-beta 1 was also observed in 
      the malignant tumors, but only the extracellular form of the protein was present; 
      intracellular TGF-beta 1 was not detected in these tumors. The expression of 
      TGF-beta 1 in this carcinogenesis model seems to have two formal stages, the 
      first being an overexpression step as a reaction to the uncontrolled growth and 
      the second being one in which tumors have no internal expression of TGF-beta 1 
      but in which external protein accumulates in the surrounding stroma. A possible 
      explanation of this paradox may be that TGF-beta 1 has functions other than its 
      growth-repressing activity.
FAU - Zenklusen, J C
AU  - Zenklusen JC
AD  - University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, 
      Smithville 78957.
FAU - Stockman, S L
AU  - Stockman SL
FAU - Fischer, S M
AU  - Fischer SM
FAU - Conti, C J
AU  - Conti CJ
FAU - Gimenez-Conti, I B
AU  - Gimenez-Conti IB
LA  - eng
GR  - RR5511/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Carcinog
JT  - Molecular carcinogenesis
JID - 8811105
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene
MH  - Animals
MH  - Cheek
MH  - Cricetinae
MH  - Disease Models, Animal
MH  - Extracellular Space/chemistry
MH  - Immunohistochemistry
MH  - Intracellular Fluid/chemistry
MH  - Male
MH  - Mesocricetus
MH  - Mouth Neoplasms/*chemically induced/genetics/*physiopathology
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics
MH  - Skin Neoplasms/chemically induced/genetics/physiopathology
MH  - Transforming Growth Factor beta/analysis/genetics/*physiology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/mc.2940090104 [doi]
PST - ppublish
SO  - Mol Carcinog. 1994 Jan;9(1):10-6. doi: 10.1002/mc.2940090104.