PMID- 8299961
OWN - NLM
STAT- MEDLINE
DCOM- 19940308
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 137
IP  - 2
DP  - 1993 Dec 31
TI  - A telomere-like satellite (GGGTCAT)n comprises 4% of genomic DNA of Drosophila 
      hydei and is located mainly in centromeric heterochromatin of all large 
      acrocentric autosomes.
PG  - 287-91
AB  - We describe the isolation and analysis of a G+C-rich telomere-like (GGGTCAT)n 
      satellite of Drosophila hydei. GGGTCAT-specific clones were obtained by a 
      combination of restriction enzyme digestion and fractionation of D. hydei DNA by 
      pulsed-field gel electrophoresis (PFGE). High-molecular-weight DNA (> 50 kb) was 
      recovered from gels, sheared by sonication and cloned into the pBS plasmid 
      vector. The results of PFGE analysis, quantitative dot blot measurements and 
      fluorescence in situ hybridization (FISH) on metaphase chromosomes show that the 
      tandemly arranged GGGTCAT sequences comprise 4% of genomic DNA and are organized 
      as megabase-sized clusters in the pericentric region of all large acrocentric 
      autosomes. A telomere-related function is rather unlikely, because the 
      chromosomal ends of D. hydei seem to be free of GGGTCAT repeats and several other 
      species of Drosophila do not contain any cross-hybridizing sequences at all.
FAU - Burgtoft, C
AU  - Burgtoft C
AD  - Department of Biochemistry, University of Oxford, UK.
FAU - Bunemann, H
AU  - Bunemann H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Centromere
MH  - Cloning, Molecular
MH  - DNA, Satellite/*genetics
MH  - Drosophila/*genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - *Heterochromatin
MH  - Molecular Sequence Data
MH  - *Telomere
EDAT- 1993/12/31 00:00
MHDA- 1993/12/31 00:01
CRDT- 1993/12/31 00:00
PHST- 1993/12/31 00:00 [pubmed]
PHST- 1993/12/31 00:01 [medline]
PHST- 1993/12/31 00:00 [entrez]
AID - 0378-1119(93)90022-U [pii]
AID - 10.1016/0378-1119(93)90022-u [doi]
PST - ppublish
SO  - Gene. 1993 Dec 31;137(2):287-91. doi: 10.1016/0378-1119(93)90022-u.