PMID- 8300158
OWN - NLM
STAT- MEDLINE
DCOM- 19940310
LR  - 20191023
IS  - 0882-0139 (Print)
IS  - 0882-0139 (Linking)
VI  - 22
IP  - 8
DP  - 1993 Dec
TI  - Nitric oxide mediates the toxoplasmastatic activity of murine microglial cells in 
      vitro.
PG  - 487-501
AB  - Toxoplasma gondii (T. gondii), an opportunistic protozoan, is an important cause 
      of central nervous system (CNS) infections in immunosuppressed patients. The 
      present study focused on the interaction between T. gondii and microglial cells 
      from the brain of neonatal Balb/c mice. Preincubation of the murine microglial 
      cells with recombinant interferon-gamma (rIFN-gamma) and lipopolysaccharide (LPS) 
      induced significant inhibition of T. gondii replication in a dose dependent 
      manner. This antiparasitic effect in microglial cells was correlated with the 
      induction of the L-arginine-dependent generation of reactive nitrogen 
      intermediates (RNIs). Tumor necrosis factor-alpha (TNF-alpha) was also involved 
      in the toxoplasmastatic activity. Microglial cells incubated with recombinant 
      TNF-alpha in combination with a non-activating concentration of rIFN-gamma 
      released substantial amount of RNIs. Neutralizing antibodies against mouse 
      TNF-alpha inhibited the release of RNI by rIFN-gamma activated macrophages. In 
      summary, the present results show that activation of microglial cells by 
      rIFN-gamma and LPS induce the production of nitric oxide (NO) by these cells via 
      an L-arginine dependent pathway. NO appears to be the effector molecule mediating 
      the toxoplasmastatic effects in these cells.
FAU - Jun, C D
AU  - Jun CD
AD  - Department of Microbiology/Immunology, School of Medicine, Wonkwang University, 
      Korea.
FAU - Kim, S H
AU  - Kim SH
FAU - Soh, C T
AU  - Soh CT
FAU - Kang, S S
AU  - Kang SS
FAU - Chung, H T
AU  - Chung HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Arginine/physiology
MH  - Brain/cytology/*immunology/parasitology
MH  - Cells, Cultured
MH  - Fluorescent Antibody Technique
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microglia/*immunology/parasitology
MH  - Nitric Oxide/*physiology
MH  - Recombinant Proteins
MH  - Toxoplasma/*immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.3109/08820139309084178 [doi]
PST - ppublish
SO  - Immunol Invest. 1993 Dec;22(8):487-501. doi: 10.3109/08820139309084178.