PMID- 8300755
OWN - NLM
STAT- MEDLINE
DCOM- 19940307
LR  - 20201209
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 53
IP  - 4
DP  - 1993 Dec
TI  - Immunohistochemical localization of the avian progesterone receptor and its 
      candidate receptor binding factor (RBF-1).
PG  - 383-93
AB  - An avian oviduct nuclear matrix protein in the 6-10 kDa size range has been 
      implicated to function in the cell-free nuclear binding of the avian oviduct 
      progesterone receptor (PR). This protein, termed the receptor binding factor-1 
      (RBF-1), has been purified and partially characterized [Schuchard et al.: 
      Biochemistry 30:4535-4542, 1991]. This paper describes the immunohistochemical 
      co-localization of the RBF-1 and PR in the avian oviduct cell nuclei and rat 
      reproductive cell nuclei using antibodies directed specifically against the RBF-1 
      and activated PR. In the undifferentiated oviduct, the immunoreactivities for 
      both PR and RBF-1 were co-localized in the nuclei of only epithelial cells, but 
      not the stromal cells or smooth muscle cells. In the partially differentiated 
      oviduct of estrogen treated chicks, the immunoreactivity co-localized in the 
      nuclei of not only epithelial but also glandular and stromal cells. Staining for 
      the PR, but not RBF-1, was detected in the smooth muscle cells. The intensity of 
      the PR but not the RBF-1 staining was markedly down-regulated in these cells at 2 
      and 6 h after treatment of the animals with progesterone (P). However, the band 
      patterns for RBF-1 in the Western blots did show qualitative changes which may 
      reflect P-induced posttranslational modifications which alter the epitope on the 
      RBF-1. Interestingly, immunohistochemical analysis of several reproductive 
      tissues of the rat showed that certain cell types in the uterus, ovary, and 
      prostate displayed strong positive nuclear staining for an RBF-1-like 
      antigen(s).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Zhuang, Y H
AU  - Zhuang YH
AD  - Department of Biomedical Sciences, University of Tampere, Finland.
FAU - Landers, J P
AU  - Landers JP
FAU - Schuchard, M D
AU  - Schuchard MD
FAU - Syvala, H
AU  - Syvala H
FAU - Gosse, B
AU  - Gosse B
FAU - Ruesink, T
AU  - Ruesink T
FAU - Spelsberg, T C
AU  - Spelsberg TC
FAU - Tuohimaa, P
AU  - Tuohimaa P
LA  - eng
GR  - HD 07108/HD/NICHD NIH HHS/United States
GR  - HD9140/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Avian Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RBF-1 protein, Gallus gallus)
RN  - 0 (Receptors, Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.1.38 (trans-2-enoyl-CoA reductase (NADPH))
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - *Avian Proteins
MH  - Blotting, Western
MH  - Carrier Proteins/*analysis
MH  - Cell Nucleus/chemistry
MH  - *Chickens
MH  - Chromatin/chemistry
MH  - DNA-Binding Proteins/*analysis
MH  - Epithelium/chemistry
MH  - Estradiol/pharmacology
MH  - Female
MH  - Immunohistochemistry
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Molecular Sequence Data
MH  - Oviducts/*chemistry/ultrastructure
MH  - Oxidoreductases
MH  - Oxidoreductases Acting on CH-CH Group Donors
MH  - Rats
MH  - Receptors, Progesterone/*analysis
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1002/jcb.240530416 [doi]
PST - ppublish
SO  - J Cell Biochem. 1993 Dec;53(4):383-93. doi: 10.1002/jcb.240530416.