PMID- 8300859
OWN - NLM
STAT- MEDLINE
DCOM- 19940308
LR  - 20190825
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 50
IP  - 1
DP  - 1994 Feb
TI  - Beta-endorphin modulates calcium channel activity in human neutrophils.
PG  - 77-83
AB  - 10(-6) M n-formyl-methionyl-leucyl-phenylalanine (FMLP) stimulated Ca2+ flux in 
      human neutrophils is characterized by a profile composed of two peaks of 
      different amplitude and breadth. beta-Endorphin inhibited the magnitude and 
      modulated the kinetics of the second peak in a manner which was dose-dependent 
      and could reflect either negative cooperativity or heterogeneity of binding 
      sites. The second peak arises from calcium channel activity since in the presence 
      of nifedipine or EGTA it was not evident while the first peak was reduced about 
      24%. Similarly, at 15 degrees C, where we were unable to detect any channel 
      activity, the first peak was diminished by 35% and beta-endorphin had no 
      detectable effect on this peak. These results led us to conclude that the first 
      peak is chiefly composed of Ca2+ recruited from cytosolic stores which are 
      relatively insensitive to the above treatments and a smaller fraction of calcium 
      originating in calcium channel activity. Hence, we reason that beta-endorphin 
      modulates only the calcium ion flux arising from calcium channel function.
FAU - Mazorow, D L
AU  - Mazorow DL
AD  - New Ideas Co., Rockville, MD 20852.
FAU - Simpkins, C O
AU  - Simpkins CO
FAU - Millar, D B
AU  - Millar DB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Calcium Channels)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 60617-12-1 (beta-Endorphin)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Calcium Channels/*drug effects/physiology
MH  - Cytosol/metabolism
MH  - Egtazic Acid/pharmacology
MH  - Humans
MH  - Neutrophils/*drug effects/metabolism
MH  - Nifedipine/pharmacology
MH  - Temperature
MH  - beta-Endorphin/*pharmacology
EDAT- 1994/02/01 00:00
MHDA- 2000/06/01 09:00
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 0165-5728(94)90217-8 [pii]
AID - 10.1016/0165-5728(94)90217-8 [doi]
PST - ppublish
SO  - J Neuroimmunol. 1994 Feb;50(1):77-83. doi: 10.1016/0165-5728(94)90217-8.