PMID- 8301133
OWN - NLM
STAT- MEDLINE
DCOM- 19940309
LR  - 20220318
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 152
IP  - 3
DP  - 1994 Feb 1
TI  - Macrophage inflammatory protein-1 alpha and monocyte chemoattractant peptide-1 
      elicit immediate and late cutaneous reactions and activate murine mast cells in 
      vivo.
PG  - 1298-303
AB  - We have previously reported that monocyte chemoattractant protein-1 (MCP-1) is 
      the most potent histamine-releasing factor (HRF) for basophils. Macrophage 
      inflammatory protein-1 alpha (MIP-1 alpha) has modest histamine-releasing 
      activity. The objective of this study was to investigate whether MCP-1 and MIP-1 
      alpha would activate mast cells in vivo and induce a cutaneous inflammatory 
      reaction in mice. To this goal, mouse hind footpads were separately injected with 
      20 microliters of human recombinant MCP-1 or MIP-1 alpha (10(-7) M). Diluent was 
      used as a control in the second footpad. The footpad-swelling response was 
      measured at 30 min, 1 h, and then hourly for 6 h. Both MCP-1 (2.72 +/- 0.2 vs 2.1 
      +/- 0.03 mm for diluent, n = 8, p < 0.02) and MIP-1 alpha (3.0 +/- 0.1 vs 2.1 +/- 
      0.03 mm for diluent, n = 8, p < 0.02) induced an immediate swelling reaction. The 
      immediate reaction was followed by a sustained late reaction that peaked within 1 
      h and lasted for more than 6 h. Histologic examination of the footpads, obtained 
      at hour 2, revealed that MCP-1 caused mild mononuclear cell infiltrates, moderate 
      degranulation of mast cells, and soft tissue swelling. In contrast, MIP-1 alpha 
      induced a severe inflammatory reaction that consisted of neutrophils, mononuclear 
      cells, and degranulated mast cells. Electron microscope examination of the tissue 
      revealed features of extensive mast cell degranulation by MIP-1 alpha and to a 
      lesser extent by MCP-1. Thus, we conclude that mast cells are activated on 
      injection of MCP-1, whereas degranulation of mast cells and recruitment of 
      leukocytes contribute to the footpad reaction induced with MIP-1 alpha.
FAU - Alam, R
AU  - Alam R
AD  - Department of Internal Medicine, University of Texas Medical Branch, Galveston 
      77555-0762.
FAU - Kumar, D
AU  - Kumar D
FAU - Anderson-Walters, D
AU  - Anderson-Walters D
FAU - Forsythe, P A
AU  - Forsythe PA
LA  - eng
GR  - A12294/PHS HHS/United States
GR  - A127864/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
SB  - IM
MH  - Animals
MH  - Cell Degranulation
MH  - Chemokine CCL2
MH  - Chemokine CCL4
MH  - Chemotactic Factors/*immunology
MH  - Chemotaxis, Leukocyte
MH  - Cytokines/*immunology
MH  - Immunity, Cellular
MH  - Macrophage Inflammatory Proteins
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron
MH  - Monokines/*immunology
MH  - Skin Tests
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1994 Feb 1;152(3):1298-303.