PMID- 8302123
OWN - NLM
STAT- MEDLINE
DCOM- 19940310
LR  - 20071114
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 104
IP  - 2
DP  - 1994 Feb
TI  - Cytokines in experimental otitis media with effusion.
PG  - 191-6
AB  - Studies in the authors' laboratory have recently demonstrated the presence of 
      potent inflammatory cytokines such as interleukin-1 beta (IL-1 beta), 
      interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF alpha) in human middle 
      ear effusions. The clinical significance of this finding has not been fully 
      elucidated because of the limitations of human studies. We hypothesized that the 
      chinchilla model of otitis media may be an appropriate system with which to study 
      the role of cytokines in otitis media with effusion. To begin to investigate this 
      possibility, 30 chinchillas underwent surgical blockage of the eustachian tube 
      (ET) to promote effusion development. After 2 weeks, examination by otoscopy 
      demonstrated 27 ears to have developed an effusion. Next, all middle ear clefts, 
      in random manner, were either injected with heat-killed Streptococcus pneumoniae 
      1 x 10(6) in 0.1 mL normal saline, injected with 0.1 mL normal saline alone, or 
      received no injection at all. Middle ear effusions were obtained and analyzed for 
      IL-1 beta and TNF alpha by enzyme-linked immunosorbent assay (ELISA). This study 
      demonstrated a significant correlation between IL-1 beta and the presence of an 
      effusion (P < .001). Additionally, increased TNF alpha levels correlated with 
      bacterial component presence (P < .001), i.e., mean TNF alpha level was 108, 
      10.8, and 0 pg/mL in bacteria, normal saline, and noninjected ears, respectively. 
      These findings would suggest that cytokine expression may relate to specific 
      pathological conditions and that the chinchilla model for otitis media with 
      effusion (OME) could be used to further explore the role of cytokines in OME.
FAU - Johnson, M D
AU  - Johnson MD
AD  - Department of Surgery, University of Connecticut Health Center, Farmington 06032.
FAU - Fitzgerald, J E
AU  - Fitzgerald JE
FAU - Leonard, G
AU  - Leonard G
FAU - Burleson, J A
AU  - Burleson JA
FAU - Kreutzer, D L
AU  - Kreutzer DL
LA  - eng
GR  - EY04131/EY/NEI NIH HHS/United States
GR  - HL25015/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chinchilla
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Interleukin-1/*metabolism
MH  - Macrophages/metabolism
MH  - Otitis Media with Effusion/immunology/*metabolism/microbiology
MH  - Pneumococcal Infections/immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1288/00005537-199402000-00012 [doi]
PST - ppublish
SO  - Laryngoscope. 1994 Feb;104(2):191-6. doi: 10.1288/00005537-199402000-00012.