PMID- 8302296
OWN - NLM
STAT- MEDLINE
DCOM- 19940304
LR  - 20190825
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 31
IP  - 1
DP  - 1994 Jan
TI  - Induction of phosphatidylinositol turnover and EGR-1 mRNA expression by 
      crosslinking of surface IgM and IgD in the human B cell line B104.
PG  - 21-30
AB  - We have previously shown that a human B lymphoma cell line, B104, expressed 
      surface IgM (sIgM) and surface IgD (sIgD), and that crosslinking of sIgM and sIgD 
      by anti-IgM antibody (Ab) and anti-IgD Ab, respectively, induced Ca2+ influx to 
      almost the same degree, whereas only sIgM-crosslinking caused B104 cell death. 
      Here, we investigated the accumulation of cyclic AMP (cAMP), the hydrolysis of 
      inositol phosphates, protein kinase C (PKC) activity and the induction of Egr-1 
      and c-fos mRNA expression by sIgM- and sIgD-crosslinking to examine differences 
      in the signals mediated through sIgM and sIgD in B104 cells. Both sIgM- and 
      sIgD-crosslinking with antibodies induced elevation of cAMP levels, 
      phosphatidylinositol turnover, PKC activation and expression of Egr-1 and c-fos 
      mRNA, although sIgM-crosslinking was more effective than sIgD-crosslinking, 
      presumably due to the higher expression of sIgM than of sIgD. Egr-1 mRNA 
      expression induced by sIgM- and sIgD-crosslinking was inhibited by H7, erbstatin 
      and genistein, but not by HA1004. Erbstatin and genistein inhibited the 
      sIg-crosslinking-induced Egr-1 mRNA expression in a dose-dependent manner 
      parallel to that observed in the inhibition of sIg-crosslinking-induced protein 
      tyrosine phosphorylation. Phorbol myristate acetate induced Egr-1 mRNA expression 
      but forskolin and dibutyryl cyclic AMP did not. These findings suggest that the 
      Egr-1 mRNA activating signals through sIgM and sIgD are protein tyrosine kinase- 
      and PKC-dependent, but protein kinase A-independent. Cyclosporin A (CsA) and 
      FK506 rescued B104 cells from death induced by anti-IgM Ab, but did not affect 
      the expression of Egr-1 and c-fos mRNA, showing that CsA and FK506 affect signal 
      transducers differently from or downstream to these molecules. The difference in 
      signals transduced through sIgM and sIgD in B104 cells is discussed.
FAU - Kanazashi, S
AU  - Kanazashi S
AD  - Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
FAU - Hata, D
AU  - Hata D
FAU - Ishigami, T
AU  - Ishigami T
FAU - Jung, E Y
AU  - Jung EY
FAU - Shintaku, N
AU  - Shintaku N
FAU - Sumimoto, S
AU  - Sumimoto S
FAU - Heike, T
AU  - Heike T
FAU - Katamura, K
AU  - Katamura K
FAU - Mayumi, M
AU  - Mayumi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EGR1 protein, human)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Transcription Factors)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
GS  - c-fos
GS  - c-myc
MH  - B-Lymphocytes/immunology/*metabolism
MH  - Cross-Linking Reagents
MH  - Cyclic AMP/metabolism
MH  - DNA-Binding Proteins/biosynthesis/*genetics
MH  - Early Growth Response Protein 1
MH  - Genes, fos
MH  - Genes, myc
MH  - Humans
MH  - *Immediate-Early Proteins
MH  - Immunoglobulin D/*metabolism
MH  - Immunoglobulin M/*metabolism
MH  - Phosphatidylinositols/*metabolism
MH  - Protein Kinase C/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Antigen, B-Cell/*metabolism
MH  - Signal Transduction/drug effects
MH  - Transcription Factors/biosynthesis/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1016/0161-5890(94)90134-1 [doi]
PST - ppublish
SO  - Mol Immunol. 1994 Jan;31(1):21-30. doi: 10.1016/0161-5890(94)90134-1.